Snezana Drmanac, Radoje Drmanac, Donald Swanson, and Steve Huang are employed by Callida Genomics, Inc. (Sunnyvale, CA), a company that is developing a commercial HyChip™ system. In addition, Snezana Drmanac and Radoje Drmanac are officers of Callida Genomics, Inc.
Article first published online: 27 JUL 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 24, Issue 3, pages 261–271, September 2004
How to Cite
Cowie, S., Drmanac, S., Swanson, D., Delgrosso, K., Huang, S., du Sart, D., Drmanac, R., Surrey, S. and Fortina, P. (2004), Identification of APC gene mutations in colorectal cancer using universal microarray-based combinatorial sequencing-by-hybridization. Hum. Mutat., 24: 261–271. doi: 10.1002/humu.20078
Communicated by Arupa Ganguly
The Supplementary Material referred to in this article can be found at http://www.interscience.wiley.com/jpages/1059-7794/suppmat
- Issue published online: 27 JUL 2004
- Article first published online: 27 JUL 2004
- Manuscript Accepted: 15 APR 2004
- Manuscript Received: 4 FEB 2004
- National Institutes of Health (NIH). Grant Numbers: R33-CA83220, HL69256
- National Institute of Standards and Technology (NIST)
- Advanced Technology Program. Grant Number: Project ID 200-00-4467A
- colorectal cancer;
- mutation detection;
Familial adenomatous polyposis (FAP) is an autosomal dominant inherited form of colorectal cancer, caused mostly by mutations in the APC gene. Due to the wide variety of mutations found and the large size of the APC gene, several methods of mutation detection are used, which can be time consuming and costly. Here we demonstrate a new method of mutation detection in the APC gene using an array-based approach termed combinatorial sequencing-by-hybridization (cSBH). In cSBH, a universal probe set is attached to a support and a second one is in solution. Two-probe ligation occurs when a DNA strand from the target PCR product consecutively anneals to both unlabeled array-bound and solution-phase dye-labeled probe, creating all target-complementary long-labeled probes attached to the surface. A standard array reader scores fluorescent signals at each array position. Cell lines and patient DNA with known APC gene mutations were analyzed using a cSBH-based HyChip™ product. Results show that this universal hexamer (6-mer) chip can successfully detect a range of mutations. Results are very robust for a continuous readout of 3.6 kb from a PCR target, with 99.97% accuracy on a single HyChip™ slide. cSBH is a fast, cost-efficient method for first stage mutation screening in the APC or any other gene. Hum Mutat 24:261–271, 2004. © 2004 Wiley-Liss, Inc.