Communicated by Christine Van Broeckhoven
Comprehensive analysis of LRRK2 in publicly available Parkinson's disease cases and neurologically normal controls
Article first published online: 22 JAN 2008
This article is a US Government work, and, as such, is in the public domain in the United States of America. Published in 2008 by Wiley-Liss, Inc.
Special Issue: Focus on Pharmacogenetics
Volume 29, Issue 4, pages 485–490, April 2008
How to Cite
Paisán-Ruíz, C., Nath, P., Washecka, N., Gibbs, J. R. and Singleton, A. B. (2008), Comprehensive analysis of LRRK2 in publicly available Parkinson's disease cases and neurologically normal controls. Hum. Mutat., 29: 485–490. doi: 10.1002/humu.20668
This article is a US Government work, and, as such, is in the public domain in the United States of America.
- Issue published online: 10 MAR 2008
- Article first published online: 22 JAN 2008
- Manuscript Revised: 21 SEP 2007
- Manuscript Accepted: 21 SEP 2007
- Manuscript Received: 6 JUL 2007
- Intramural Program, National Institute on Aging, National Institutes of Health, Department of Health and Human Services
- Neurotoxin Exposure Treatment Research Program (NETRP), Department of Defense. Grant Number: W81XWH-06-1-0792
- association studies;
- linkage disequilibrium;
- tagging SNPs;
- Parkinson's disease
Mutation of LRRK2, encoding dardarin, is the most common known genetic cause of Parkinson's disease (PD). The large size of this gene and the relative ease with which the most common mutations can be screened means that although more than 50 LRRK2 screening papers have been published, few have analyzed the entire coding sequence. Furthermore, no comprehensive sequence-based analysis has been performed on control samples. Here, we present sequencing of all coding exons in a series of 275 PD cases and 275 neurologically normal controls and analysis of the LRRK2 locus for whole gene multiplications or deletions. We also present case–control SNP association results using 74 SNPs genotyped across LRRK2. We identified six novel disease-associated missense mutations, including two that altered the same residue of the protein. These data and analysis of previously reported disease-segregating mutations shows that the majority of disease-causing mutations lie in the C-terminal half of the protein. Hum Mutat 29(4), 485–490, 2008. Published 2008, Wiley-Liss, Inc.