Communicated by Mauno Vihinen
Article first published online: 15 APR 2008
This article is a US Government work, and, as such, is in the public domain in the United States of America. Published in 2008 by Wiley-Liss, Inc.
Volume 29, Issue 6, pages 861–868, June 2008
How to Cite
Lopez-Granados, E., Keenan, J. E., Kinney, M. C., Leo, H., Jain, N., Ma, C. A., Quinones, R., Gelfand, E. W. and Jain, A. (2008), A novel mutation in NFKBIA/IKBA results in a degradation-resistant N-truncated protein and is associated with ectodermal dysplasia with immunodeficiency. Hum. Mutat., 29: 861–868. doi: 10.1002/humu.20740
This article is a US Government work, and, as such, is in the public domain in the United States of America
- Issue published online: 22 MAY 2008
- Article first published online: 15 APR 2008
- Manuscript Accepted: 24 DEC 2007
- Manuscript Received: 29 JUN 2007
- primary immunodeficiency;
- ectodermal dysplasia;
- lymphocyte activation;
- antigen presenting cell;
Alterations in nuclear factor kappa B (NF-κB) essential modulator (NEMO; HUGO-approved symbol IKBKG) underlie most cases of ectodermal dysplasia with immune deficiency (EDI), a human disorder characterized by anhidrosis with diminished immunity. EDI has also been associated with a single heterozygous mutation at position Ser32 of the NF-κB inhibitor IκBα, one of two phosphorylation sites that are essential for targeting IκBα for proteasomal degradation and hence for activation of NF-κB. We report a novel heterozygous nonsense mutation in the IKBA (HUGO-approved symbol, NFKBIA) gene of a 1-year-old male child with EDI that introduces a premature termination codon at position Glu14. An in-frame methionine downstream of the nonsense mutation allows for reinitiation of translation. The resulting N-terminally truncated protein lacks both serine phosphorylation sites and inhibits NF-κB signaling by functioning as a dominant negative on NF-κB activity in lymphocytes and monocytes. These findings support the scanning model for translation initiation in eukaryotes and confirm the critical role of the NF-κB in the human immune response. Hum Mutat 29(6), 861–868, 2008. Published 2008, Wiley-Liss, Inc.