MicroSNiPer: a web tool for prediction of SNP effects on putative microRNA targets
Article first published online: 7 OCT 2010
© 2010 Wiley-Liss, Inc.
Volume 31, Issue 11, pages 1223–1232, November 2010
How to Cite
Barenboim, M., Zoltick, B. J., Guo, Y. and Weinberger, D. R. (2010), MicroSNiPer: a web tool for prediction of SNP effects on putative microRNA targets. Hum. Mutat., 31: 1223–1232. doi: 10.1002/humu.21349
- Issue published online: 28 OCT 2010
- Article first published online: 7 OCT 2010
- Accepted manuscript online: 31 AUG 2010 12:00AM EST
- Manuscript Accepted: 5 AUG 2010
- Manuscript Received: 2 APR 2010
- FASTA program;
- gene expression;
- computational prediction
MicroRNAs are short, approximately 22 nucleotide noncoding RNAs binding to partially complementary sites in the 3′UTR of target mRNAs. This process generally results in repression of multiple targets by a particular microRNA. There is substantial interest in methods designed to predict the microRNA targets and effect of single nucleotide polymorphisms (SNPs) on microRNA binding, given the impact of microRNA on posttranscriptional regulation and its potential relation to complex diseases. We developed a web-based application, MicroSNiPer, which predicts the impact of a SNP on putative microRNA targets. This application interrogates the 3′-untranslated region and predicts if a SNP within the target site will disrupt/eliminate or enhance/create a microRNA binding site. MicroSNiPer computes these sites and examines the effects of SNPs in real time. MicroSNiPer is a user-friendly Web-based tool. Its advantages include ease of use, flexibility, and straightforward graphical representation of the results. It is freely accessible at http://cbdb.nimh.nih.gov/microsniper. Hum Mutat 31:–11, 2010. © 2010 Wiley-Liss, Inc.