Communicated by Mario Tosi
Alternative Splicing of In-Frame Exon Associated with Premature Termination Codons: Implications for Readthrough Therapies
Article first published online: 8 NOV 2012
© 2012 Wiley Periodicals, Inc.
Volume 34, Issue 2, pages 287–291, February 2013
How to Cite
Hinzpeter, A., Aissat, A., de Becdelièvre, A., Bieth, E., Sondo, E., Martin, N., Costes, B., Costa, C., Goossens, M., Galietta, L. J.V., Girodon, E. and Fanen, P. (2013), Alternative Splicing of In-Frame Exon Associated with Premature Termination Codons: Implications for Readthrough Therapies. Hum. Mutat., 34: 287–291. doi: 10.1002/humu.22236
Contract grant sponsors: Institut National de la Santé et de la Recherche Médicale (INSERM); «Vaincre la Mucoviscidose».
- Issue published online: 29 JAN 2013
- Article first published online: 8 NOV 2012
- Accepted manuscript online: 15 OCT 2012 03:56AM EST
- Manuscript Accepted: 1 OCT 2012
- Manuscript Received: 13 FEB 2012
- Institut National de la Santé et de la Recherche Médicale (INSERM)
- cystic fibrosis;
- premature termination codon;
The correction of premature termination codons (PTCs) by agents that promote readthrough represents a promising emerging tool for the treatment of many genetic diseases. The efficiency of the treatment, however, varies depending on the stop codon itself and the amount of correctible transcripts related to the efficiency of nonsense-mediated decay. In the current study, a screen by in vitro minigene assay of all six PTCs described in exon 15 of the CFTR gene demonstrated alternative splicing to differing degrees for five of them. Of the five, PTC mutations c.2537G>A (p.Trp846*UAG) and c.2551C>T (p.Arg851*) cause the greatest proportion of transcripts lacking exon 15; both mutations altering exonic splicing regulatory elements. In order to increase the amount of full-length transcripts, different pharmacological treatments were performed showing both negative and positive effects on exon inclusion for the same mutation. Therefore, the total amount of transcripts together with the splicing profile should be assessed to anticipate and improve efficacy of readthrough therapy.