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humu22426-sup-0001-FigureS1.pdf152KSupp. Figure S1. The 40 amino acid N-terminal tag on PMS2 does not influence MMR activity. A. Representative expression of 35S-Methionine-labeled proteins, visualized after SDS-PAGE gel electrophoresis and autoradiography. B. Repair efficiencies of in vitro produced PMS2 with or without fused N-terminal tags. PMS2 proteins were sequentially diluted twofold and dimerized to MLH1. Repair assays were carried out as described. MMR-proficient HeLa nuclear extract serves as a positive control. No significant differences were found between any of the PMS2 measurements, supporting the robustness of the assay. Results are shown as mean±S.E.M. of 4 independent experiments.

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