Contract grant sponsor: NIH (1R01CA164944-01A1).
Inactivation of DNA Mismatch Repair by Variants of Uncertain Significance in the PMS2 Gene
Version of Record online: 11 SEP 2013
© 2013 WILEY PERIODICALS, INC.
Volume 34, Issue 11, pages 1477–1480, November 2013
How to Cite
Drost, M., Koppejan, H. and de Wind, N. (2013), Inactivation of DNA Mismatch Repair by Variants of Uncertain Significance in the PMS2 Gene. Hum. Mutat., 34: 1477–1480. doi: 10.1002/humu.22426
Communicated by Rolf H. Sijmons
- Issue online: 9 OCT 2013
- Version of Record online: 11 SEP 2013
- Accepted manuscript online: 20 AUG 2013 08:38AM EST
- Manuscript Accepted: 5 AUG 2013
- Manuscript Received: 3 JUN 2013
- NIH. Grant Number: 1R01CA164944-01A1
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|humu22426-sup-0001-FigureS1.pdf||152K||Supp. Figure S1. The 40 amino acid N-terminal tag on PMS2 does not influence MMR activity. A. Representative expression of 35S-Methionine-labeled proteins, visualized after SDS-PAGE gel electrophoresis and autoradiography. B. Repair efficiencies of in vitro produced PMS2 with or without fused N-terminal tags. PMS2 proteins were sequentially diluted twofold and dimerized to MLH1. Repair assays were carried out as described. MMR-proficient HeLa nuclear extract serves as a positive control. No significant differences were found between any of the PMS2 measurements, supporting the robustness of the assay. Results are shown as mean±S.E.M. of 4 independent experiments.|
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