Identification of novel SNPs in the interleukin 6 receptor gene (IL6R)

Authors

  • Lyoung Hyo Kim,

    1. Department of Genetic Epidemiology, SNP Genetics, Inc., 11th Floor, Maehun B/D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110-834, Korea
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  • Hyo-Suk Lee,

    Corresponding author
    1. Department of Internal Medicine, Seoul National University college of Medicine 28 Yungun-dong, Chongro-Gu Seoul, Korea
    • Department of Internal Medicine, Seoul National University Hospital. 28 Yungun-dong, Chongro-Gu, Seoul, Korea 110-744
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  • Yoon Jun Kim,

    1. Department of Internal Medicine, Seoul National University college of Medicine 28 Yungun-dong, Chongro-Gu Seoul, Korea
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  • Ji Hyun Jung,

    1. Department of Genetic Epidemiology, SNP Genetics, Inc., 11th Floor, Maehun B/D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110-834, Korea
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  • Jun Yeun Kim,

    1. Department of Genetic Epidemiology, SNP Genetics, Inc., 11th Floor, Maehun B/D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110-834, Korea
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  • Byung Lae Park,

    1. Department of Genetic Epidemiology, SNP Genetics, Inc., 11th Floor, Maehun B/D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110-834, Korea
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  • Hyoung Doo Shin

    1. Department of Genetic Epidemiology, SNP Genetics, Inc., 11th Floor, Maehun B/D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110-834, Korea
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Abstract

The human interleukin-6 receptor (IL6R) is responsible for signal transduction of IL6 that might have associations with immune diseases and various infectious diseases. We have sequenced all 10 exons including the putative splicing site to identify single nucleotide polymorphisms in IL6R. Seven novel single nucleotide polymorphisms (SNPs) were identified; one SNP in the promoter region (−183G>A), one synonymous SNP in exon 2 (24013G>A: Ala31Ala), one non-synonymous SNP in exon 9 (48892A>C: Asp358Ala) and four in introns (29753A>C, 42700T>C, 48869T>A and 59818C>T). The frequencies of each SNP in the Korean population (n=300) were 0.48 (-183 G>A), 0.13 (24013 G>A), 0.41 (29753A>C), 0.41 (42700T>C), 0.1 (48869T>A), 0.42 (48892A>C), and 0.07 (59818C>T), respectively. Haplotypes and their frequencies were estimated by the EM algorithm. Linkage disequilibrium coefficients (∣D'∣) between each SNP pair were also calculated. The information on SNPs and haplotypes in IL6R would be useful for genetic studies of this gene. © 2003 Wiley-Liss, Inc.

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