Serum lipopolysaccharide-binding protein in endotoxemic patients with inflammatory bowel disease
Article first published online: 19 DEC 2006
Copyright © 2006 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 13, Issue 3, pages 269–277, March 2007
How to Cite
Pastor Rojo, Ó., López San Román, A., Albéniz Arbizu, E., de la Hera Martínez, A., Ripoll Sevillano, E. and Albillos Martínez, A. (2007), Serum lipopolysaccharide-binding protein in endotoxemic patients with inflammatory bowel disease. Inflamm Bowel Dis, 13: 269–277. doi: 10.1002/ibd.20019
- Issue published online: 16 FEB 2007
- Article first published online: 19 DEC 2006
- Manuscript Accepted: 25 SEP 2006
- Manuscript Received: 19 JUL 2006
- Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III. Grant Number: CM03/00041
- inflammatory bowel disease;
- Crohn's disease;
- ulcerative colitis;
- bacterial translocation;
- lipopolysaccharide-binding protein
Background: In inflammatory bowel disease (IBD), enhanced inflammatory activity in the gut is thought to increase the risk of bacterial translocation and endotoxemia. By searching for signs of endotoxin-signaling cascade activation, including augmented levels of endotoxin, lipopolysaccharide-binding protein (LBP), and soluble CD14 receptor (sCD14), this prospective study sought to establish whether endotoxemia could contribute to greater clinical activity of disease.
Methods: Concentrations of plasma endotoxin, LBP, sCD14, several cytokines, acute phase proteins and clinical activity indices were determined in 104 patients with Crohn's disease (CD) and 52 patients with ulcerative colitis (UC).
Results: Endotoxemia was present in 48% of the patients with CD and in 28% of the patients with UC. The mean LBP was higher in patients with active CD (23.1 ± 13.7 μg/mL) and UC (21.4 ± 10.9 μg/mL) than in healthy controls (7.2 ± 1.8 μg/mL; P < 0.01). Elevated serum concentrations of endotoxin and LBP were even detected in patients with inactive CD. Among the patients with active IBD, those with higher endotoxin levels had the worst clinical activity scores and the highest LBP levels. Treatment normalized LBP concentrations, from 29.1 ± 13.0 to 15.2 ± 7.3 μg/mL; (P < 0.05) in active CD and from 21.7 ± 9.8 to 13.6 ± 5.7 μg/mL; (P < 0.01) in active UC, along with normalizing endotoxin and sCD14 plasma concentrations.
Conclusions: Patients with IBD show increased serum levels of endotoxin, LBP and sCD14. This alteration correlates with disease activity, with normal levels recovered after treatment, although less completely in Crohn's disease, and parallels a rise in proinflammatory cytokines, suggesting a contribution of bacterial products to the inflammatory cascade in these patients.
(Inflamm Bowel Dis 2007)