Apoptosis and Bax, Bcl-2, Mcl-1 expression in neutrophils of Crohn's disease patients
Article first published online: 11 FEB 2008
Copyright © 2008 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 14, Issue 6, pages 819–825, June 2008
How to Cite
Catarzi, S., Marcucci, T., Papucci, L., Favilli, F., Donnini, M., Tonelli, F., Vincenzini, M. T. and Iantomasi, T. (2008), Apoptosis and Bax, Bcl-2, Mcl-1 expression in neutrophils of Crohn's disease patients. Inflamm Bowel Dis, 14: 819–825. doi: 10.1002/ibd.20397
- Issue published online: 5 MAY 2008
- Article first published online: 11 FEB 2008
- Manuscript Accepted: 20 DEC 2007
- Manuscript Received: 11 JUN 2007
- Donation from the Compagnia San Paolo (Torino)
- Fondazione Cassa di Risparmio di Firenze
- Fondazione IBD Onlus (Torino)
- Crohn's disease;
- polymorphonuclear neutrophils;
- Bcl-2 family protein expression
Background: The etiology of Crohn's disease (CD) remains unknown, and the defective function of neutrophils appears to be associated with this pathology. Neutrophils undergo spontaneous apoptosis which, if not tightly regulated, can induce the development of chronic inflammatory disease. The Bcl-2 protein family is also involved in the regulation of neutrophil apoptosis.
Methods: This study investigated the apoptosis and expression of some regulatory factors in CD patient and control polymorphonuclear neutrophils (PMN) in suspension and in adhesion on fibronectin, an extracellular matrix protein. These 2 conditions mimic circulating neutrophils before they are recruited at the intestinal levels, and their adhesion to tissue.
Results: Apoptosis in CD patient PMN was delayed in suspension and accelerated in adhesion, which is the opposite of what happens in controls. Higher levels of Bax, Bcl-2, and Mcl-1 proteins were registered in freshly isolated CD patient PMN, in contrast to controls, in which Bcl-2 protein was undetectable. Among the studied pro- and antiapoptotic factors, Bax levels seem to be mainly related to the difference in apoptosis between PMN of CD patients and controls.
Conclusions: For the first time it has been demonstrated by direct experimental evidence that apoptosis in CD patient PMN is regulated differently from that of control PMN. Abnormal expression of regulating apoptosis proteins is shown in CD patient PMN. These data suggest that the defective functionality of neutrophils can be the early event responsible for the altered mucosal immune response in CD, and that neutrophil apoptosis may offer a new target for specific drugs and therapy tools.
(Inflamm Bowel Dis 2008)