Does nicotine influence cytokine profile and subsequent cell cycling/apoptotic responses in inflammatory bowel disease?
Article first published online: 10 JUL 2008
Copyright © 2008 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 14, Issue 11, pages 1469–1482, November 2008
How to Cite
Aldhous, M. C., Prescott, R. J., Roberts, S., Samuel, K., Waterfall, M. and Satsangi, J. (2008), Does nicotine influence cytokine profile and subsequent cell cycling/apoptotic responses in inflammatory bowel disease?. Inflamm Bowel Dis, 14: 1469–1482. doi: 10.1002/ibd.20523
- Issue published online: 15 OCT 2008
- Article first published online: 10 JUL 2008
- Manuscript Accepted: 2 MAY 2008
- Manuscript Received: 14 DEC 2007
- National Association for Crohn's and Colitis
- Chief Scientist's Office of the Scottish Executive
- cell cycle;
- inflammatory bowel disease
Background: Smoking differentially influences susceptibility to the inflammatory bowel diseases (IBDs) Crohn's disease (CD) and ulcerative colitis (UC). We investigated the effects of nicotine on cytokine, cell cycle, and apoptotic responses in peripheral blood mononuclear cells (PBMCs) from IBD patients and healthy controls (HCs).
Methods: PBMCs from IBD patients and HC were stimulated with lipopolysaccharide (LPS; 1 μg/mL) or phytohemagglutinin (PHA, 5 or 0.5 μg/mL), ± nicotine (1, 10, 100 μg/mL). Cytokines (IL1β, IL2, IL10, IL12/IL23p40, TGFβ, TNFα) were measured in supernatants at 24 hours. After 72 hours cells were analyzed by flow cytometry for cell cycle and apoptosis. Statistical modeling was used to identify interactions between cytokines and cell cycle / apoptosis and minimize confounding effects.
Results: Stimulation by LPS and PHA (5 μg/mL) increased IL12/IL23p40 production from CD and UC versus HC (P < 0.05); PHA (0.5 μg/mL) increased IL1β in UC and decreased TGFβ from CD and UC (P < 0.01). In all groups, nicotine reduced LPS- and PHA (0.5 μg/mL)-stimulated production of IL1β, IL10, TGFβ, and TNFα (P < 0.001). Cell cycle analysis showed that PHA, but not LPS, induced proliferation and decreased G0/G1 resting cells in CD and UC versus HC (P < 0.001). Nicotine decreased PHA-stimulated S-phase proliferation and increased G0/G1 resting cells (P < 0.01). Modeling showed independent associations between IL12/IL23p40 and apoptosis (P = 0.01), IL1β and resting cells (P = 0.006), TNFα and proliferating cells (P < 0.001). Disease activity and smoking habit had no effect.
Conclusions: Dysregulated cytokine profiles in UC and CD are associated with specific alterations in cell cycle responses; these effects may be modified by nicotine, and potentially by anticytokine therapies.
(Inflamm Bowel Dis 2008)