Supported, in part, by funding for the IPODD (Intestinal Proteases: Opportunity for Drug Discovery) consortium through Coordination Theme 1 (Health) of the European Union's FP7, Grant agreement number HEALTH-F2-2008-202020. FS and the Alimentary Pharmabiotic Centre is supported by a research centre grant from Science Foundation Ireland.
Degradation of the extracellular matrix components by bacterial-derived metalloproteases: Implications for inflammatory bowel diseases†
Article first published online: 17 SEP 2010
Copyright © 2010 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 17, Issue 5, pages 1189–1200, May 2011
How to Cite
Pruteanu, M., Hyland, N. P., Clarke, D. J., Kiely, B. and Shanahan, F. (2011), Degradation of the extracellular matrix components by bacterial-derived metalloproteases: Implications for inflammatory bowel diseases. Inflamm Bowel Dis, 17: 1189–1200. doi: 10.1002/ibd.21475
- Issue published online: 11 APR 2011
- Article first published online: 17 SEP 2010
- Manuscript Accepted: 2 AUG 2010
- Manuscript Received: 31 JUL 2010
- bacterial metalloproteases;
- inflammatory bowel diseases
Proteolytic degradation of the extracellular matrix, a feature of mucosal homeostasis and tissue renewal, also contributes to the complications of intestinal inflammation. Whether this proteolytic activity is entirely host-derived, or, in part, produced by the gut microbiota, is unknown.
We screened the bacterial colonies for gelatinolytic activity from fecal samples of 20 healthy controls, 23 patients with ulcerative colitis, and 18 with Crohn's disease (CD). In addition, the genes encoding metalloproteases were detected by conventional or real-time polymerase chain reaction (PCR).
Gelatinolytic activity was found in approximately one-quarter of samples regardless of the presence of inflammation and without any attempt to enhance the sensitivity of the culture-based screen. This was associated with a diversity of bacteria, particularly in CD, but was predominantly linked with Clostridium perfringens. Culture supernatants from C. perfringens degraded gelatin, azocoll, type I collagen, and basement membrane type IV collagen, but different isolates varied in the degree of proteolytic activity. Results were confirmed by detection of the C. perfringens colA gene (encoding collagenase) in fecal DNA, again regardless of the presence or absence of inflammation. However, the biologic significance and potential implications of microbial-derived proteolytic activity were confirmed by reduced transepithelial resistance (TER) after exposure of rat distal colon to culture supernatants of C. perfringens in Ussing chambers.
The study shows that microbial-derived proteolytic activity has the capacity to contribute to mucosal homeostasis and may participate in the pathogenesis of inflammatory bowel disease. (Inflamm Bowel Dis 2010)