B cells exposed to enterobacterial components suppress development of experimental colitis
Article first published online: 25 MAY 2011
Copyright © 2011 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 18, Issue 2, pages 284–293, February 2012
How to Cite
Schmidt, E. G. W., Larsen, H. L., Kristensen, N. N., Poulsen, S. S., Claesson, M. H. and Pedersen, A. E. (2012), B cells exposed to enterobacterial components suppress development of experimental colitis. Inflamm Bowel Dis, 18: 284–293. doi: 10.1002/ibd.21769
- Issue published online: 10 JAN 2012
- Article first published online: 25 MAY 2011
- Manuscript Accepted: 12 APR 2011
- Manuscript Received: 6 APR 2011
- Danish Agency for Science, Technology and Innovation
- Colitis Crohn's Foundation
- Aage and Johanne Louis-Hansen Foundation
- Aase and Ejnar Danielsen Foundation
- Augustinus Foundation
- Lundbeck Foundation
- Regulatory B cells;
- colitis, IL-10;
B cells positively contribute to immunity by antigen presentation to CD4+ T cells, cytokine production, and differentiation into antibody secreting plasma cells. Accumulating evidence implies that B cells also possess immunoregulatory functions closely linked to their capability of IL-10 secretion.
Colitis development was followed in CD4+CD25− T cell transplanted SCID mice co-transferred with B cells exposed to an enterobacterial extract (ebx-B cells). B and T cell cytokine expression was measured by flow cytometry and enzyme-linked immunosorbent assay (ELISA).
We demonstrate that splenic B cells exposed to ebx produce large amounts of IL-10 in vitro and express CD1d and CD5 previously known to be associated with regulatory B cells. In SCID mice transplanted with colitogenic CD4+CD25− T cells, co-transfer of ebx-B cells significantly suppressed development of colitis. Suppression was dependent on B cell-derived IL-10, as co-transfer of IL-10 knockout ebx-B cells failed to suppress colitis. Ebx-B cell-mediated suppression of colitis was associated with a decrease in interferon gamma (IFN-γ)-producing TH1 cells and increased frequencies of Foxp3-expressing T cells.
These data demonstrate that splenic B cells exposed to enterobacterial components acquire immunosuppressive functions by which they can suppress development of experimental T cell-mediated colitis in an IL-10-dependent way. (Inflamm Bowel Dis 2011;)