Anti-inflammatory properties of dairy lactobacilli

Authors

  • Clarissa Santos Rocha MSc,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
    3. Department of General Biology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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    • C. Santos Rocha is the recipient of a fellowship in the framework of the EC EST project “LABHEALTH,” Marie Curie Contract MEST-2-CT-2004-514428, and the Brazilian funding agency CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior). This work was also supported by FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brasil) and CNPq (Conselho Nacional de Desenvolvimento Científicoe Tecnológico, Brasil).

  • Omar Lakhdari PhD,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Hervé M. Blottière PhD,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Sébastien Blugeon,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Harry Sokol PhD,

    1. Gastroenterology and Nutrition Department, Saint Antoine Hospital, Paris, France
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  • Luis G. Bermúdez-Humarán PhD,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Vasco Azevedo PhD,

    1. Department of General Biology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • Anderson Miyoshi PhD,

    1. Department of General Biology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • Joël Doré PhD,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Philippe Langella PhD,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Emmanuelle Maguin PhD,

    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
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  • Maarten van de Guchte PhD

    Corresponding author
    1. INRA, UMR1319 Micalis, Jouy en Josas, France
    2. AgroParisTech, UMR Micalis, Jouy-en-Josas, France
    • Institut Micalis (Microbiologie de l'Alimentation au service de la Santé), INRA (UMR1319) et AgroParisTech, Domaine de Vilvert, F-78352 Jouy-en-Josas, cedex, France
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Abstract

Background:

The intestinal microbiota plays an important role in human health through the modulation of innate immune responses. While selected commensal bacteria are marketed in specific probiotic products to control these responses, relatively little is known about the immune modulation potential of dairy bacteria that have principally been selected for their fermentation properties. The modulation of innate immune responses may reduce chronic inflammation in inflammatory bowel diseases like ulcerative colitis.

Methods:

A collection of dairy Lactobacillus delbrueckii strains was screened for immune modulation effects in vitro through the quantification of nuclear factor kappa B (NF-κB) activation in a human intestinal epithelial cell line. Selected bacterial strains were then tested in vivo in a mouse dextran sodium sulfate (DSS) colitis model.

Results:

All L. delbrueckii strains tested showed anti-inflammatory effects in vitro, to an extent that varied between strains. These effects rely on bacterial surface exposed proteins and affect the central part of the NF-κB activation pathway. One of the selected strains significantly reduced the macroscopic and microscopic symptoms of DSS-induced colitis in the mouse intestinal tract, diminished body weight loss, and improved survival.

Conclusions:

The results of this study show that dairy lactobacilli that often are part of a regular diet can modulate innate immune responses, and may thus affect health more than generally thought. One of the strains tested alleviated the symptoms of DSS-induced colitis in mice, a model of human ulcerative colitis. (Inflamm Bowel Dis 2011;)

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