The first two authors contributed equally to this work.
AIEC colonization and pathogenicity: Influence of previous antibiotic treatment and preexisting inflammation†
Article first published online: 16 FEB 2012
Copyright © 2012 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 18, Issue 10, pages 1923–1931, October 2012
How to Cite
Drouet, M., Vignal, C., Singer, E., Djouina, M., Dubreuil, L., Cortot, A., Desreumaux, P. and Neut, C. (2012), AIEC colonization and pathogenicity: Influence of previous antibiotic treatment and preexisting inflammation. Inflamm Bowel Dis, 18: 1923–1931. doi: 10.1002/ibd.22908
Supported by IRMAD/Astrazeneca, Association François Aupetit, HFSP.
- Issue published online: 13 SEP 2012
- Article first published online: 16 FEB 2012
- Manuscript Accepted: 16 JAN 2012
- Manuscript Received: 30 DEC 2011
Additional supporting information may be found in the online version of this article.
|IBD_22908_sm_SuppFig1.TIF||56K||Supporting Figure 1: Study design. (A) WT and NOD2KO mice were orally given antibiotics (ATB) once a day for three days. At the end of the 3th day of antibiotic treatment, mice were orally challenged with 109 CFU of E. coli AIEC (strain LF82 or strain 06362), once a day for two days. Animals were harvested at day 1,5 and 60 after infection. (B) WT mice were given 2% (wt/vol) of dextran sulfate sodium (DSS), for 9 days, in drinking water starting with the antibiotic treatment previously described, and were challenged with AIEC 06362 or E. coli K12 Animals were harvested after 9 days of DSS treatment.|
|IBD_22908_sm_SuppFig2.TIF||82K||Supporting Figure 2: AIEC LF82 colonization and persistence in the ileum and colon after antibiotic treatment. Quantification of ileal (A) or colonic (B) mucosal-associated AIEC in WT and NOD2KO mice receiving antibiotic treatment (ATB) at day 1 (D1), 5 (D5) and 60 (D60) after oral infection with 109 AIEC LF82. Results are expressed as log CFU per gram of tissue with a detection threshold of 4 log CFU/g. Number of mice per group: n= 4 to 6 in the WT and NOD2KO AIEC LF82-infected and n=6 in the other groups. Error bars represent SEM. *, p < 0.05; **, p < 0.01.|
|IBD_22908_sm_SuppFig3.TIF||80K||Supporting Figure 3: AIEC LF82 translocation to the mesenteric lymph nodes and mesenteric fat after antibiotic treatment. Quantification of AIEC LF82 bacteria in the mesenteric lymph nodes (MLN) (A) or mesenteric fat (B) of WT and NOD2KO mice receiving antibiotic treatment at day 1 (D1), 5 (D5) and 60 (D60) after oral infection with 109 AIEC LF82. Results are expressed as log CFU per gram of tissue with a detection threshold of 2 log CFU/g. Number of mice per group :n= 4 to 6 in the WT and NOD2KO AIEC LF82-infected and n=6 in the other groups. Error bars represent SEM. *, p < 0.05; **, p < 0.01.|
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