The first two authors contributed equally to this work.
Defective tumor necrosis factor release from Crohn's disease macrophages in response to toll-like receptor activation: Relationship to phenotype and genome-wide association susceptibility loci†
Article first published online: 20 MAR 2012
Copyright © 2012 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 18, Issue 11, pages 2120–2127, November 2012
How to Cite
Sewell, G. W., Rahman, F. Z., Levine, A. P., Jostins, L., Smith, P. J., Walker, A. P., Bloom, S. L., Segal, A. W. and Smith, A. M. (2012), Defective tumor necrosis factor release from Crohn's disease macrophages in response to toll-like receptor activation: Relationship to phenotype and genome-wide association susceptibility loci. Inflamm Bowel Dis, 18: 2120–2127. doi: 10.1002/ibd.22952
Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms
- Issue published online: 15 OCT 2012
- Article first published online: 20 MAR 2012
- Manuscript Accepted: 17 FEB 2012
- Manuscript Received: 10 FEB 2012
- Wellcome Trust. Grant Number: (grant GHACB)
- National Association of Colitis and Crohn's disease
Additional Supporting Information may be found in the online version of this article.
|IBD_22952_sm_SuppFig1.tif||488K||Supporting Information Figure 1. Signalling downstream of TLR2 and the resultant pro-inflammatory gene induction are indistinguishable between CD and control macrophages. (A) Phosphorylation of p38 MAP kinase and JNK, and degradation of I?B-α in macrophages from CD (n=7) and HC (n=5) subjects in response to stimulation with PAM3. Representative western blots from individual subjects are shown on the right. (B) mRNA profiles of genes related to TLR signalling in CD (n=9) and HC (n=8) macrophages after stimulation with PAM3. There were no significant differences between macrophages from CD and HC subjects.|
|IBD_22952_sm_SuppFig2.tif||10105K||Supporting Information Figure 2. Supernatants from CD macrophages stimulated for 6 h with A. PAM3CSK4 and B. LPS; and the amount of TNF released was measured and correlated with age. Results for TNF against age are expressed as scatter plots with the corresponding correlation (R2).|
|IBD_22952_sm_SuppTab1.doc||64K||Supporting Information Table 1. Details for the 34 CD-associated SNPs included. The potential candidate genes of interest for each locus are those reported by Franke et al. (4)|
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