Additional Supporting Information may be found in the online version of this article.

IBD_22952_sm_SuppFig1.tif488KSupporting Information Figure 1. Signalling downstream of TLR2 and the resultant pro-inflammatory gene induction are indistinguishable between CD and control macrophages. (A) Phosphorylation of p38 MAP kinase and JNK, and degradation of I?B-α in macrophages from CD (n=7) and HC (n=5) subjects in response to stimulation with PAM3. Representative western blots from individual subjects are shown on the right. (B) mRNA profiles of genes related to TLR signalling in CD (n=9) and HC (n=8) macrophages after stimulation with PAM3. There were no significant differences between macrophages from CD and HC subjects.
IBD_22952_sm_SuppFig2.tif10105KSupporting Information Figure 2. Supernatants from CD macrophages stimulated for 6 h with A. PAM3CSK4 and B. LPS; and the amount of TNF released was measured and correlated with age. Results for TNF against age are expressed as scatter plots with the corresponding correlation (R2).
IBD_22952_sm_SuppTab1.doc64KSupporting Information Table 1. Details for the 34 CD-associated SNPs included. The potential candidate genes of interest for each locus are those reported by Franke et al. (4)

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