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Additional Supporting Information may be found in the online version of this article.

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IBD_22992_sm_SuppFig1.TIF308KSupporting Information Figure 1. Schematic representation of the STAT-signaling pathway from cytokine stimulation of the receptor till gene expression. Cytokine binding to the receptor induces its dimerization and phosphorylation of Janus kinases (JAK) by the intrinsic tyrosine kinase activity. The activated JAK phosphorylates tyrosine residues that are then able to bind STAT proteins. Next, JAK phosphorylates STAT enabling its dimerization and translocation to the nucleus. In the nucleus the STAT-dimers bind to the DNA and recruit cofactors like the transcription Factor II D (TFIID) to start transcription of STAT-target genes. One of these target genes, the suppressor of cytokine signaling (SOCS) is able to negatively regulate STAT activation by binding to the phophorylted tyrosine residues on the receptor.
IBD_22992_sm_SuppFig2.TIF76KSupporting Information Figure 2. The percentage of SOCS3 positive IEC was assessed in expression biopsies from the baseline colonoscopy during remission. The SOCS3 expression in IEC from rectum biopsies was compared between individuals who had a histological relapse (A) within 1-3, 4-6, 7-9 years or who did not relapse during follow up (no > 9) (Kruskal-Wallis test p=0.0027; 1-3(y) vs. 7-9(y), p=0.0159; 1-3(y) vs. no >9(y), p=0.0068; 4-6(y) vs. no >9(y), p=0.0104). The SOCS3 expression in IEC from rectum biopsies was compared between individuals who had an endoscopic (B) or clinical (C) relapse within 1-4, 5-9 years or who did not relapse during follow up (no > 9) (endoscopic: Kruskal-Wallis test p=0.0014; 1-4(y) vs. no >9(y), p=0.0010; clinical: Kruskal-Wallis test p=0.0120; 1-4(y) vs. no >9(y), p=0.0057). (D). The STAT3 expression in IEC from rectum biopsies was generally low and showed no differences between the histological relapse group within 1-3, 4-6, 7-9 years and who did not relapse during follow up (no > 9).
IBD_22992_sm_SuppFig3.TIF62KSupporting Information Figure 3. No (0), mild (1), moderate (2) or high (3) basal plasmacytosis in descending colon biopsies taken during baseline colonoscopy was expressed for each patient relapsing within 4 years after the biopsy was taken (A). The percentage of SOCS3 positive IEC in these biopsies is expressed for all patients with similar basal plasmacytosis (B).
IBD_22992_sm_SuppFig4.TIF602KSupporting Information Figure 4. Normal biopsy staining Biopsies of normal colon were stained for SOCS3, p-STAT3 and p-STAT1 as control (x 400 and x 2400 inserts).

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