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Enhanced expression of CXCL10 in inflammatory bowel disease: Potential role of mucosal toll-like receptor 3 stimulation

Authors

  • Ann E. Østvik MD,

    1. Department of Gastroenterology and Hepatology, St. Olav's University Hospital, Trondheim, Norway
    2. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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    • The first two authors contributed equally to this work

  • Atle vB Granlund MSc,

    1. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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    • The first two authors contributed equally to this work

  • Marit Bugge MSc,

    1. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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  • Nadra J. Nilsen PhD,

    1. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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  • Sverre H. Torp MD, PhD,

    1. Department of Pathology and Medical Genetics, St. Olav's University Hospital, Trondheim, Norway
    2. Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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  • Helge L. Waldum MD, PhD,

    1. Department of Gastroenterology and Hepatology, St. Olav's University Hospital, Trondheim, Norway
    2. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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  • Jan K. Damås MD, PhD,

    1. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    2. Department of Infectious Diseases, St. Olav's University Hospital, Trondheim, Norway
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  • Terje Espevik PhD,

    1. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
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  • Arne K. Sandvik MD, PhD

    Corresponding author
    1. Department of Gastroenterology and Hepatology, St. Olav's University Hospital, Trondheim, Norway
    2. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    • Department of Cancer Research and Molecular Medicine, Faculty of Medicine NTNU, P.O. Box 8905, N-7491 Trondheim, Norway
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Abstract

Background:

We explored the gene expression in colonic biopsies of active and inactive inflammatory bowel disease (IBD) in an extensive material of ulcerative colitis (UC) and Crohn's disease (CD). The chemokine CXCL10 and its receptor CXCR3 were among the upregulated genes. This study examined the expression of CXCL10 and the mechanisms for its release in patients with UC or CD and in intestinal epithelial cell (IEC) lines.

Methods:

A microarray gene expression analysis was done on colonic biopsies (n = 133) from patients with IBD. Biopsies were studied with immunohistochemistry for CXCL10 and CXCR3 expression. Mechanisms for CXCL10 release in peripheral blood mononuclear cells (PBMCs) and in the colonic epithelial cell lines HT-29 and SW620 were studied upon pattern recognition receptor (PRR) stimulation.

Results:

CXCL10 and CXCR3 mRNA abundances were increased in biopsies from active UC and CD compared to inactive disease and controls. CXCL10 was mainly localized to mucosal epithelial cells, with increased immunostaining in active IBD. CXCR3-positive cells were scattered in the lamina propria. CXCL10 was secreted from the colonic epithelial cell lines in response to the Toll-like receptor 3 (TLR3) ligand polyinosinic:polycytidylic acid (poly(I:C)). This ligand also induced a marked release of CXCL10 in PBMCs from IBD patients and controls.

Conclusions:

We identified CXCL10 and CXCR3 as upregulated genes in colonic mucosa in active IBD. The TLR3-ligand poly(I:C) markedly increased release of CXCL10 in colonic epithelial cell lines, suggesting a TLR3-mediated CXCL10 release from mucosal epithelial cells in IBD patients. (Inflamm Bowel Dis 2012;)

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