This paper is dedicated to Tom CJ Feijtel (1959–2005), who inspired many of us.
Animal use replacement, reduction, and refinement: Development of an integrated testing strategy for bioconcentration of chemicals in fish†
Article first published online: 5 NOV 2009
Copyright © 2007 SETAC
Integrated Environmental Assessment and Management
Volume 3, Issue 1, pages 3–17, January 2007
How to Cite
de Wolf, W., Comber, M., Douben, P., Gimeno, S., Holt, M., Léonard, M., Lillicrap, A., Sijm, D., van Egmond, R., Weisbrod, A. and Whale, G. (2007), Animal use replacement, reduction, and refinement: Development of an integrated testing strategy for bioconcentration of chemicals in fish. Integr Environ Assess Manag, 3: 3–17. doi: 10.1002/ieam.5630030102
- Issue published online: 5 NOV 2009
- Article first published online: 5 NOV 2009
- Manuscript Accepted: 23 MAR 2006
- Manuscript Received: 24 NOV 2005
- Integrated testing strategy;
- Animal testing;
- OECD 305
When addressing the use of fish for the environmental safety of chemicals and effluents, there are many opportunities for applying the principles of the 3Rs: Reduce, Refine, and Replace. The current environmental regulatory testing strategy for bioconcentration and secondary poisoning has been reviewed, and alternative approaches that provide useful information are described. Several approaches can be used to reduce the number of fish used in the Organization for Economic Cooperation and Development (OECD) Test Guideline 305, including alternative in vivo test methods such as the dietary accumulation test and the static exposure approach. The best replacement approach would seem to use read-across, chemical grouping, and quantitative structure-activity relationships with an assessment of the key processes in bioconcentration: Adsorption, distribution, metabolism, and excretion. Biomimetic extraction has particular usefulness in addressing bioavailable chemicals and is in some circumstances capable of predicting uptake. Use of alternative organisms such as invertebrates should also be considered. A single cut-off value for molecular weight and size beyond which no absorption will take place cannot be identified. Recommendations for their use in bioaccumulative (B) categorization schemes are provided. Assessment of biotransformation with in vitro assays and in silico approaches holds significant promise. Further research is needed to identify their variability and confidence limits and the ways to use this as a basis to estimate bioconcentration factors. A tiered bioconcentration testing strategy has been developed taking account of the alternatives discussed.