You have full text access to this Open Access content

Immunity, Inflammation and Disease

Cover image for Vol. 1 Issue 1

October 2013

Volume 1, Issue 1

Pages i–ii, 1–69

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Original Research
    5. Review
    1. You have full text access to this OnlineOpen article
      Issue Information (pages i–ii)

      Version of Record online: 29 OCT 2013 | DOI: 10.1002/iid3.15

  2. Editorial

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Original Research
    5. Review
    1. You have full text access to this OnlineOpen article
  3. Original Research

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Original Research
    5. Review
    1. You have full text access to this OnlineOpen article
      Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo (pages 3–13)

      Nitya Nair, Ludovico Buti, Elisa Faenzi, Francesca Buricchi, Sandra Nuti, Chiara Sammicheli, Simona Tavarini, Maximilian W.L. Popp, Hidde Ploegh, Francesco Berti, Mariagrazia Pizza, Flora Castellino, Oretta Finco, Rino Rappuoli, Giuseppe Del Giudice, Grazia Galli and Monia Bardelli

      Version of Record online: 1 AUG 2013 | DOI: 10.1002/iid3.3

      Thumbnail image of graphical abstract

      We describe two independent methods to optimize protein antigen labeling with fluorochromes for FACS identification and analysis of rare antigen-specific memory B cells: conventional amine labeling with stringently controlled reaction parameters, and a novel site-specific method, named sortagging, where nucleophilic fluorochromes are added in known numbers to LPTEG motifs expressed on the target proteins by using the staphylococcal sortase A. Using the meningococcal serogroup B NadA antigen and a mouse model of vaccination, we show that both methods permit the specific identification of all NadA-specific memory B cells by FACS directly in ex vivo samples. We demonstrate that both techniques minimize the degree of labeling while maintaining adequate signal to noise ratios for sensitive and identification of rare memory B cells specific for at least two different antigens. This method facilitates accurate control of antigen integrity thus providing the potential for more valid analysis and sorting of antigen-specific B cell repertoires and a powerful tool for best understanding B cell dynamics following vaccination or infection.

    2. You have full text access to this OnlineOpen article
      Agonistic anti-CD40 promotes early development and increases the incidence of severe thyroid epithelial cell hyperplasia (TEC H/P) in CD4−/− mice (pages 14–25)

      Shiguang Yu, Edward F. Downey and Helen Braley-Mullen

      Version of Record online: 10 SEP 2013 | DOI: 10.1002/iid3.5

      Thumbnail image of graphical abstract

      Thyrocyte hyperplasia is an autoimmune disease characterized by uncontrolled proliferation of thyroid epithelial cells (called TEC H/P). CD4/IFNg−/− NOD.H-2h4 mice can develop severe TEC H/P, while CD8/IFNg−/− mice are resistant. Although splenocytes from mice with severe TEC H/P generally transfer TEC H/P to SCID recipients, splenocytes from CD4−/− mice could not transfer TECH/P unless donors were given agonistic anti-CD40. Thus anti-CD40 provides an important signal for activation of autoreactive CD8 cells that transfer TEC H/P.

    3. You have full text access to this OnlineOpen article
      Human pre-B cell receptor signal transduction: evidence for distinct roles of PI3kinase and MAP-kinase signalling pathways (pages 26–36)

      Kolandaswamy Anbazhagan, Amrathlal Rabbind Singh, Piec Isabelle, Ibata Stella, Alleaume-De Martel Céline, Eliane Bissac, Brassart Bertrand, Nyga Rémy, Taylor Naomi, Fuentes Vincent, Jacques Rochette and Kaïss Lassoued

      Version of Record online: 24 SEP 2013 | DOI: 10.1002/iid3.4

      Thumbnail image of graphical abstract

      PI3K and MAPK exerted opposing effects on the pre-BCR-induced activation of the canonical NF-κB and c-Fos/AP1 pathways. In addition, pre-BCR-induced down-regulation of Rag1, Rag2, E2A and Pax5 transcripts occurred in a PI3K-dependent manner.

    4. You have full text access to this OnlineOpen article
      Enhanced expression of the soluble form of E-selectin attenuates progression of lupus nephritis and vasculitis in MRL/lpr mice (pages 37–46)

      Kimihiko Nakatani, Shuhei Yoshimoto, Osamu Asai, Hirokazu Sakan, Miho Terada, Yoshihiko Saito, Masato Nose, Masayuki Iwano and Noboru Konishi

      Version of Record online: 25 SEP 2013 | DOI: 10.1002/iid3.6

      Thumbnail image of graphical abstract

      This study provides the first evidence that E-selectin plays a critical role in the progression of lupus nephritis and soluble form of E-selectin proteins may enable development of a new approach to the treatment of lupus nephritis.

    5. You have full text access to this OnlineOpen article
      Relative contribution of IL-1α, IL-1β and TNF to the host response to Mycobacterium tuberculosis and attenuated M. bovis BCG (pages 47–62)

      Marie-Laure Bourigault, Noria Segueni, Stéphanie Rose, Nathalie Court, Rachel Vacher, Virginie Vasseur, François Erard, Marc Le Bert, Irene Garcia, Yoichiro Iwakura, Muazzam Jacobs, Bernhard Ryffel and Valerie F. J. Quesniaux

      Version of Record online: 30 SEP 2013 | DOI: 10.1002/iid3.9

      Thumbnail image of graphical abstract

      Here, we confirm that both TNF and IL-1 pathways are required to control M. tuberculosis infection since absence of both IL-1a and IL-1ß recapitulated the dramatic defect seen in the absence of IL-1R1 or TNF. However, presence of either IL-1a or IL-1ß allows some control of acute M. tuberculosis infection. Further, although TNF is essential for the early control of infection by either virulent or attenuated mycobacteria, IL-1 pathway is dispensable for controlling less virulent infection by M. bovis BCG.

  4. Review

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Original Research
    5. Review
    1. You have full text access to this OnlineOpen article
      The use of prebiotics during the first year of life for atopy prevention and treatment (pages 63–69)

      Priscilla Negrão de Moura and Nelson Augusto Rosário Filho

      Version of Record online: 25 SEP 2013 | DOI: 10.1002/iid3.8

      Thumbnail image of graphical abstract

      The incidence of allergic diseases has increased in recent decades. We searched the MEDLINE, EMBASE, Cochrane Library, LILACS, SciELO, IBECS, Web of Science and Clinical Trials databases as well as Google Scholar and the references of the articles identified. Although there was a trend for reduced allergic symptoms following the administration of prebiotics, there was not sufficient evidence to establish that such treatment is effective for the prevention of allergies in children.

SEARCH

SEARCH BY CITATION