A pilot study showing differences in glycosylation patterns of IgG subclasses induced by pneumococcal, meningococcal, and two types of influenza vaccines

Authors

  • Anne Cathrine Vestrheim,

    Corresponding author
    1. Department of Bacteriology & Immunology, Norwegian Institute of Public Health, Oslo, Norway
    2. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway
    • Correspondence

      Anne Cathrine Vestrheim, Department of Bacteriology & Immunology, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, 0403 Oslo, Norway.

      Tel: +47-21-07-63-24;

      Fax: +47-21-07-65-18;

      E-mail: anne.cathrine.vestrheim@fhi.no

      Correspondence

      Terje Einar Michaelsen, Department of Bacteriology & Immunology, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, 0403 Oslo, Norway.

      Tel: +47-21-07-63-24;

      Fax: +47-21-07-65-18;

      E-mail: t.e.michaelsen@farmasi.uio.no

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  • Anders Moen,

    1. Department of Molecular Biosciences, University of Oslo, Oslo, Norway
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  • Wolfgang Egge-Jacobsen,

    1. Department of Molecular Biosciences, University of Oslo, Oslo, Norway
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  • Leon Reubsaet,

    1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway
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  • Trine Grønhaug Halvorsen,

    1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway
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  • Diane Bryant Bratlie,

    1. Department of Bacteriology & Immunology, Norwegian Institute of Public Health, Oslo, Norway
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  • Berit Smestad Paulsen,

    1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway
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  • Terje Einar Michaelsen

    Corresponding author
    1. Department of Bacteriology & Immunology, Norwegian Institute of Public Health, Oslo, Norway
    2. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway
    • Correspondence

      Anne Cathrine Vestrheim, Department of Bacteriology & Immunology, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, 0403 Oslo, Norway.

      Tel: +47-21-07-63-24;

      Fax: +47-21-07-65-18;

      E-mail: anne.cathrine.vestrheim@fhi.no

      Correspondence

      Terje Einar Michaelsen, Department of Bacteriology & Immunology, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, 0403 Oslo, Norway.

      Tel: +47-21-07-63-24;

      Fax: +47-21-07-65-18;

      E-mail: t.e.michaelsen@farmasi.uio.no

    Search for more papers by this author

  • Funding information
  • This work was supported by a FUGE grant from The Research Council of Norway to Glyconor, grant number 183613 and funds from the Norwegian Institute of Public Health and School of Pharmacy, University of Oslo.

Abstract

The presence of a carbohydrate moiety on asparagine 297 in the Fc part of an IgG molecule is essential for its effector functions and thus influences its vaccine protective effect. Detailed structural carbohydrate analysis of vaccine induced IgGs is therefore of interest as this knowledge can prove valuable in vaccine research and design and when optimizing vaccine schedules. In order to better understand and exploit the protective potential of IgG antibodies, we carried out a pilot study; collecting serum or plasma from volunteers receiving different vaccines and determining the IgG subclass glycosylation patterns against specific vaccine antigens at different time points using LC-ESI-MS analysis. The four vaccines included a pneumococcal capsule polysaccharide vaccine, a meningococcal outer membrane vesicle vaccine, a seasonal influenza vaccine, and a pandemic influenza vaccine. The number of volunteers was limited, but the results following immunization indicated that the IgG subclass which dominated the response showed increased galactose and the level of sialic acid increased with time for most vaccinees. Fucose levels increased for some vaccinees but in general stayed relatively unaltered. The total background IgG glycosylation analyzed in parallel varied little with time and hence the changes seen were likely to be caused by vaccination. The presence of an adjuvant in the pandemic influenza vaccine seemed to produce simpler and less varied glycoforms compared to the adjuvant-free seasonal influenza vaccine. This pilot study demonstrates that detailed IgG glycosylation pattern analysis might be a necessary step in addition to biological testing for optimizing vaccine development and strategies.

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