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iid3-sm-0001-SuppFig-S1.tif645KFigure S1. NadA labeling with SrtAStaph purification. (A) On a preparative scale 10 µm NadA-His was labeled with SrtA using either the GGG-Alexa 488 or GGG-Alexa 647 probes (2 mM). The different reactions were analyzed by SDS–PAGE to confirm labeling of NadA. (B) NadA-Alexa 488 and NadA-Alexa 647 were separated from residual unlabeled material and srtA (In) using Ni-NTA resin and the collected flow-through (FT) was dialyzed. The fractions were analyzed as described in (A).

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