Cancer Cell Biology
Mapping of two new epitopes on the apomucin encoded by MUC5AC gene: Expression in normal GI tract and colon tumors
Article first published online: 25 MAR 2002
Copyright © 2002 Wiley-Liss, Inc.
International Journal of Cancer
Volume 99, Issue 3, pages 336–343, 20 May 2002
How to Cite
Nollet, S., Forgue-Lafitte, M.-E., Kirkham, P. and Bara, J. (2002), Mapping of two new epitopes on the apomucin encoded by MUC5AC gene: Expression in normal GI tract and colon tumors. Int. J. Cancer, 99: 336–343. doi: 10.1002/ijc.10335
- Issue published online: 25 APR 2002
- Article first published online: 25 MAR 2002
- Manuscript Accepted: 11 JAN 2002
- Manuscript Revised: 7 JAN 2002
- Manuscript Received: 13 NOV 2001
- Association pour la Recherche sur le Cancer. Grant Number: 9671
- gastric mucin;
Three hybridomas secreting monoclonal antibodies (MAbs) against human (62M MAb) or rat (463M and 589M MAbs) gastric mucins were isolated. These MAbs immunoreacted against a human recombinant protein encoded by the 3′ region of the MUC5AC gene. We have mapped 2 new gastric mucin epitopes and the M1-f epitope previously characterized by the 19/21M1 MAbs on MUC5AC-encoded apomucin. The M1-f, 463/589M and 62M epitopes are located in the MUC11p15/von Willebrand factor (vWF)-A3uD4 domain, in the D4-(vWF)-like domain and in the C- and CK-vWF-like domains of MUC5AC, respectively. The 463/589M and 62M MAbs stained the surface epithelium of human gastric mucosae, but not the normal colon mucosae (except 463/589M MAbs, which immunoreacted with 5 of 49 cases). All hyperplastic polyps are stained strongly with the 463/589M MAbs and faintly with the 62M MAb. In addition, 463/589M epitope was detected in 64% of the adenomas and in 93% of the mucosae adjacent to adenocarcinomas; in contrast, only 9% of the adenomas and 29% of the mucosae adjacent to adenocarcinomas expressed the 62M epitope. The expression pattern of the 463/589M epitope in colonic carcinogenesis is different from that of the 19/21M1 epitope, although the 2 epitopes are encoded by MUC5AC gene. © 2002 Wiley-Liss, Inc.