Prognostic value of P53 mutations in rectal carcinoma
Article first published online: 14 MAY 2002
Copyright © 2002 Wiley-Liss, Inc.
International Journal of Cancer
Volume 100, Issue 2, pages 131–135, 10 July 2002
How to Cite
Rebischung, C., Gérard, J.-P., Gayet, J., Thomas, G., Hamelin, R. and Laurent-Puig, P. (2002), Prognostic value of P53 mutations in rectal carcinoma. Int. J. Cancer, 100: 131–135. doi: 10.1002/ijc.10480
- Issue published online: 11 JUN 2002
- Article first published online: 14 MAY 2002
- Manuscript Accepted: 5 APR 2002
- Manuscript Revised: 3 APR 2002
- Manuscript Received: 18 FEB 2002
- rectal carcinoma;
- p53 mutation;
- preoperative radiotherapy;
The influence of p53 mutations on the response to ionizing radiation and survival was retrospectively evaluated in patients treated with preoperative radiotherapy for rectal carcinoma. From 1989 to 1991, 86 rectal cancer patients treated by preoperative radiotherapy were included in this series. For all patients, endorectal sonography (to define ultrasonography TNM [uTNM]) was performed before treatment; 19 patients were classified as stage 1, 27 as stage 2 and 40 as stage 3. Response to radiotherapy (39 Gy in 13 fractions delivered in 17 days) was assessed by comparing the uT and the T obtained by histologic examination of the resected specimen (TNM classification). A rectal cancer biopsy was performed before treatment and enabled the search for p53 mutations by denaturing gradient gel electrophoresis (DGGE) and sequencing. The status of the p53 gene was correlated with the response to radiotherapy and survival. Forty-nine percent of the tumors presented abnormal DGGE profiles. The prevalence of p53 mutations was significantly higher in patients who did not respond to radiotherapy (63%) than in those who did respond (34%) (p < 0.01). Presence of a p53 mutation was associated with significantly shorter 5-year survival compared to patients without mutations (p < 0.02). In a multivariate analysis, p53 mutation status remained a prognostic factor independent of tumor posttreatment staging (p < 0.05). p53 status is an independent prognostic factor of response to radiotherapy and survival in rectal carcinoma. © 2002 Wiley-Liss, Inc.