Walter J. Koskinen and Ren Wei Chen contributed equally to this article.
Prevalence and physical status of human papillomavirus in squamous cell carcinomas of the head and neck
Article first published online: 6 AUG 2003
Copyright © 2003 Wiley-Liss, Inc.
International Journal of Cancer
Volume 107, Issue 3, pages 401–406, 10 November 2003
How to Cite
Koskinen, W. J., Chen, R. W., Leivo, I., Mäkitie, A., Bäck, L., Kontio, R., Suuronen, R., Lindqvist, C., Auvinen, E., Molijn, A., Quint, W. G., Vaheri, A. and Aaltonen, L.-M. (2003), Prevalence and physical status of human papillomavirus in squamous cell carcinomas of the head and neck. Int. J. Cancer, 107: 401–406. doi: 10.1002/ijc.11381
- Issue published online: 17 SEP 2003
- Article first published online: 6 AUG 2003
- Manuscript Accepted: 16 MAY 2003
- Manuscript Revised: 15 MAY 2003
- Manuscript Received: 17 DEC 2002
- Helsinki University Central Hospital Research and Education Fund
- Finnish Cancer Societies
- viral physical status;
- squamous cell carcinoma of the head and neck
Fresh-frozen biopsies were obtained from 61 patients at diagnosis of squamous cell carcinoma of the head and neck (HNSCC) for study of the prevalence and physical status of human papillomavirus (HPV) DNA. The frequency of HPV DNA and genotypes were determined by SPF10 PCR screening with a general probe hybridization and INNO-LiPA HPV genotyping assay. In addition, a single-phase PCR with primers FAP 59/64 and a nested PCR with primers CP 65/70 and CP 66/69 served to detect particularly cutaneous HPV types. By the sensitive SPF10 PCR and INNO-LiPA assay, 37 of 61 (61%) samples were positive for HPV. HPV-16 was the most frequently detected type (31 of 37, 84%). Multiple infections were found in 8 of 37 (22%) of the HPV-positive samples, and co-infection by HPV-16 and HPV-33 was predominant. No cutaneous HPV types were detected. Patients with HPV-positive tumors had similar prognosis as those with HPV-negative ones. Real-time PCR analysis of the HPV-16 positive samples indicated the presence of integrated (11 of 23, 48%), episomal (8 of 23, 35%) and mixed forms (4 of 23, 17%) of HPV DNA. The viral load of HPV DNA exhibited large variation. The median copy numbers of E6 DNA in tonsillar specimens were approximately 80,000 times higher than that in nontonsillar HNSCC ones. Patients with episomal viral DNA were more frequently found to have large (T3–T4) tumors at diagnosis than were those with integrated or mixed forms. © 2003 Wiley-Liss, Inc.