Li-Hua Jin and Qiu-Jie Shao contributed equally to this work.
Detection of point mutations of the Axin1 gene in colorectal cancers
Article first published online: 12 SEP 2003
Copyright © 2003 Wiley-Liss, Inc.
International Journal of Cancer
Volume 107, Issue 5, pages 696–699, 10 December 2003
How to Cite
Jin, L.-H., Shao, Q.-J., Luo, W., Ye, Z.-Y., Li, Q. and Lin, S.-C. (2003), Detection of point mutations of the Axin1 gene in colorectal cancers. Int. J. Cancer, 107: 696–699. doi: 10.1002/ijc.11435
- Issue published online: 14 OCT 2003
- Article first published online: 12 SEP 2003
- Manuscript Accepted: 20 JUN 2003
- Manuscript Revised: 27 MAY 2003
- Manuscript Received: 20 MAR 2003
- National Outstanding Young Investigator Award. Grant Number: 30125012
- Provincial Technology Council of Fujian. Grant Number: 2002F002
- Xiamen University Startup Fund
- Hong Kong Research Grants Council. Grant Number: HKUST6122102M
- colorectal cancers
Axin is a recently identified tumor suppressor that plays an important role in liver and colon cancers. To gain further insights into the structure and function of Axin in controlling cell growth, we analyzed 54 colorectal cancer tissues for mutations in AXIN1 gene. We employed PCR amplification with 23 sets of primers against introns that encompassed the whole coding region of AXIN1 followed by single-strand conformation polymorphism (SSCP) analysis. After subcloning and sequencing analysis of the reamplified DNA from the aberrant bands, we found, in addition to 3 silent mutations, 6 misssense point mutations in different functionally important regions. The missense mutation rate is hence 11%, suggesting that Axin deficiency may contribute to the onset of colorectal tumorigenesis. © 2003 Wiley-Liss, Inc.