A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis

Authors

  • Kosei Hasegawa,

    Corresponding author
    1. Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    2. Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    • Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
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    • Fax: +81-86-235-7193

  • Toshiro Ono,

    1. Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Hirokazu Matsushita,

    1. Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Michihide Shimono,

    1. Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Yuji Noguchi,

    1. Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Yasushi Mizutani,

    1. Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Junichi Kodama,

    1. Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Takafumi Kudo,

    1. Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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  • Eiichi Nakayama

    1. Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
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Abstract

A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries and 54 ovarian cancers of different histological types, grades and stages by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were analyzed by conventional agarose gel electrophoresis and capillary electrophoresis on a microtip device to determine the expression semiquantitatively. Little or no expression was observed in the 20 normal ovarian specimens. High AKAP3 mRNA expression was observed in 15 ovarian cancer specimens (28 %). The expression was correlated with the histological grade and clinical stage. AKAP3 mRNA was observed at a significantly higher frequency in poorly differentiated (p = 0.009) and advanced stage (III and IV, p = 0.014) tumors. No correlation was found between AKAP3 mRNA expression and other variables. In Cox multivariate analysis, AKAP3 mRNA expression was found to be a significant predictor of both overall and progression-free survival in patients with poorly differentiated tumors. © 2004 Wiley-Liss, Inc.

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