Fax: +44-20-7377-7283
Predictive Markers and Cancer Prevention
You have full text access to this OnlineOpen article
Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients
Article first published online: 28 OCT 2003
DOI: 10.1002/ijc.11596
Copyright © 2003 Wiley-Liss, Inc.
Additional Information
How to Cite
Bustin, S. A., Siddiqi, S., Ahmed, S., Hands, R. and Dorudi, S. (2004), Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients. Int. J. Cancer, 108: 412–417. doi: 10.1002/ijc.11596
Publication History
- Issue published online: 21 NOV 2003
- Article first published online: 28 OCT 2003
- Manuscript Accepted: 12 SEP 2003
- Manuscript Revised: 14 JUL 2003
- Manuscript Received: 3 JAN 2003
Funded by
- Medical Research Council (MRC)
- Bowel and Cancer Research (BCR)
- Royal Society
REFERENCES
- 1
- 2, , , , , . Prognostic factors in colorectal cancer. Literature review for clinical application. Dis Colon Rectum 1998; 41: 1033–49.
- 3, . Molecular assessment of tumour stage and disease recurrence using PCR-based assays. Mol Med Today 1998; 4: 389–96.
- 4, , , , . Tumour-derived mutated K-ras codon 12 expression in regional lymph nodes of stage II colorectal cancer patients is not associated with increased risk of cancer-related death. Int J Colorectal Dis 2001; 16: 108–11.
- 5, , , , , , , , . Genetic diagnosis of lymph-node metastasis in colorectal cancer. Lancet 1995; 345: 1257–9.
- 6, , , , , , . Micrometastases and survival in stage II colorectal cancer. N Engl J Med 1998; 339: 223–8.
- 7, , , , , , , , , , , . Use of guanylyl cyclase C for detecting micrometastases in lymph nodes of patients with colon cancer. Dis Colon Rectum 1998; 41: 310–5.
- 8, , , , , , . Spread of colorectal cancer micrometastases in regional lymph nodes by reverse transcriptase-polymerase chain reactions for carcinoembryonic antigen and cytokeratin 20. J Surg Oncol 1998; 68: 34–40.Direct Link:
- 9, , , , , , . Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients. J Clin Oncol 1998; 16: 2632–40.
- 10
- 11, , , , , , . Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. J Clin Oncol 2001; 19: 3951–9.
- 12, , , . Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients. Br J Cancer 1999; 79: 1813–20.
- 13. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000; 25: 169–93.
- 14, , , , , , , . Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 2002; 309: 293–300.
- 15. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 2002; 29: 23–39.
- 16, , , , , , , , , , . Immunohistochemical assessment of localization and frequency of micrometastases in lymph nodes of colorectal cancer. Clin Cancer Res 2002; 8: 759–67.
- 17, , , , , . Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Br J Cancer 1995; 71: 278–81.
- 18, , , , , , , , , , . Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. Ann Intern Med 1999; 131: 805–12.
- 19, , , , , , , , , , , , et al. Detection of blood-borne cells in colorectal cancer patients by nested reverse transcription-polymerase chain reaction for carcinoembryonic antigen messenger RNA: longitudinal analyses and demonstration of its potential importance as an adjunct to multiple serum markers. Cancer Res 2001; 61: 2523–32.
- 20, , , , , , . Detection of colorectal cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction for cytokeratin 20. Int J Cancer 1998; 79: 288–93.Direct Link:
- 21, , . CK20 gene expression: technical limits for the detection of circulating tumor cells. Anticancer Res 1999; 19: 2073–8.
- 22, , . Quantitative RT-PCR: pitfalls and potential. Biotechniques 1999; 26: 112–5.
- 23, , , , , , , , , , . Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group. Eur J Cancer 1998; 34: 750–3.
- 24, , , . Differential expression of carcinoembryonic antigen (CEA) splice variants in whole blood of colon cancer patients and healthy volunteers: implication for the detection of circulating colon cancer cells. Oncogene 2002; 21: 7817–23.
- 25, . The value of microarray techniques for quantitative gene profiling in molecular diagnostics. Trends Mol Med 2002; 8: 269–72.
- 26, , , , , , , , . Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction. Int J Oncol 2000; 16: 289–93.
- 27, , , . A molecular signature of metastasis in primary solid tumors. Nat Genet 2003; 33: 49–54.
- 28, , , , , , , . Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcription-polymerase chain reaction in patients with gastrointestinal or breast carcinomas. J Clin Oncol 1998; 16: 128–32.
- 29, , , . Impact of cytokeratin-20 and carcinoembryonic antigen mRNA detection by RT-PCR in regional lymph nodes of patients with colorectal cancer. Br J Cancer 2000; 83: 1323–9.
- 30, , , , , , , , , . Extensive micrometastases to lymph nodes as a marker for rapid recurrence of colorectal cancer: a study of lymphatic mapping. Clin Cancer Res 2001; 7: 1350–7.
- 31, , , , , , , . Prognostic significance of cytokeratin-20 reverse transcriptase polymerase chain reaction in lymph nodes of node-negative colorectal cancer patients. J Clin Oncol 2002; 20: 1049–55.
- 32. The cellular basis of site-specific tumor metastasis. N Engl J Med 1990; 322: 605–12.

1097-0215/asset/olbannerleft.jpg?v=1&s=45719cd7de57873027993264fcc568b335a8cd56)
1097-0215/asset/olbannerright.jpg?v=1&s=5e0fba63c1309b3036eb9215a0e1e83dd02efd19)
