Gene expression profiles in breast tumors regarding the presence or absence of estrogen and progesterone receptors

Authors

  • Maria Aparecida Nagai,

    Corresponding author
    1. Disciplina de Oncologia, Departamento de Radiologia da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
    • Disciplina de Oncologia, Departamento de Radiologia da Faculdade de Medicina da Universidade de São Paulo, Av. Dr. Arnaldo, 455, 4° Andar, CEP-01296-903, São Paulo, Brazil
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    • Fax: +55-11-3069-7292

  • Nancy da Rós,

    1. Disciplina de Oncologia, Departamento de Radiologia da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
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  • Mário Mourão Neto,

    1. Departmento de Mastologia, Hospital do Câncer A.C. Camargo, São Paulo, Brazil
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  • Silvio Rodrigues de Faria Junior,

    1. Instituto de Matemática e Estatística da Universidade de São Paulo, São Paulo, Brazil
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  • Maria Mitzi Brentani,

    1. Disciplina de Oncologia, Departamento de Radiologia da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
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  • Roberto Hirata Jr.,

    1. SENAC College of Computer Science and Technology, São Paulo, Brazil
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  • Eduardo Jordão Neves

    1. Instituto de Matemática e Estatística da Universidade de São Paulo, São Paulo, Brazil
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Abstract

Estrogen acts via its receptor (ER) to stimulate cell growth and differentiation in the mammary gland. ER and progesterone receptor (PR), which is regulated by estrogen via ER, have been used as prognostic markers in clinical management of breast cancer patients. Patients with ER breast tumors have a poorer prognosis than patients with ER+ tumors. The aim of the present study was the identification of tumor-associated genes differentially expressed in breast tumors regarding the presence or absence of ER and PR hybridized with cDNA microarrays containing 4,500 tumor-derived expressed sequence tags generated using the ORESTES technique. Samples of human primary breast carcinomas from 38 patients were analyzed. The experiments were performed in triplicates and data from each element were acquired by phosphoimage scanning. Data acquisition was performed using the ArrayVision software. After normalization statistical analysis was applied. In a preliminary analysis, 98 differentially expressed transcripts were identified, 46 were found to be more expressed in ER+/PR+ and 52 were found to be more expressed in ER/PR breast tumors. The biochemical functions of the genes in the reported expression profile are diverse and include metabolic enzymes, protein kinases, helicases, transcription factors, cell cycle regulators and apoptotic factors. ER/PR breast tumors displayed increased levels of transcripts of genes associated with neurodegeneration and genes associated with proliferation were found in ER+/PR+ tumors. © 2004 Wiley-Liss, Inc.

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