ZNF143 activates gene expression in response to DNA damage and binds to cisplatin-modified DNA

Authors

  • Hiroshi Ishiguchi,

    1. Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    2. Department of Neurology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    Search for more papers by this author
  • Hiroto Izumi,

    1. Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    Search for more papers by this author
  • Takayuki Torigoe,

    1. Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    Search for more papers by this author
  • Yoichiro Yoshida,

    1. Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    Search for more papers by this author
  • Hiroshi Kubota,

    1. HSP Research Institute, Kyoto, Japan
    Search for more papers by this author
  • Sadatoshi Tsuji,

    1. Department of Neurology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    Search for more papers by this author
  • Kimitoshi Kohno

    Corresponding author
    1. Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Fukuoka, Japan
    • Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan
    Search for more papers by this author
    • Fax: +81-93-692-2766


Abstract

We have identified a cisplatin-inducible gene, the mitochondrial ribosomal protein S11 (MRP S11) gene, by means of mRNA differential display. Functional analysis of the MRP S11 promoter showed that a Staf binding site in the promoter is required for both basal promoter activity and cisplatin-inducible activity. We also found that Staf binding activity is significantly increased in nuclear extracts from cells treated with cisplatin. ZNF 143 and ZNF 76 are human homologues of the Xenopus transcriptional activator, Staf. ZNF 143 expression is induced by cisplatin but ZNF 76 expression is not. However, ZNF 143 expression is not induced by transplatin, which is clinically ineffective. ZNF143 is an inducible gene by other DNA damaging agents such as γ-irradiation, etoposide and adriamycin. ZNF 143 also binds preferentially to cisplatin-modified DNA. These results suggest that ZNF 143 participates in cellular responses to DNA damage. © 2004 Wiley-Liss, Inc.

Ancillary