The first two authors contributed equally.
Role of p53 status in chemosensitivity determination of cancer cells against histone deacetylase inhibitor sodium butyrate
Article first published online: 1 FEB 2005
Copyright © 2005 Wiley-Liss, Inc.
International Journal of Cancer
Volume 115, Issue 1, pages 11–18, 20 May 2005
How to Cite
Joseph, J., Wajapeyee, N. and Somasundaram, K. (2005), Role of p53 status in chemosensitivity determination of cancer cells against histone deacetylase inhibitor sodium butyrate. Int. J. Cancer, 115: 11–18. doi: 10.1002/ijc.20842
- Issue published online: 18 MAR 2005
- Article first published online: 1 FEB 2005
- Manuscript Accepted: 1 OCT 2004
- Manuscript Received: 27 JUN 2004
- ICMR (Center for Advanced studies in Molecular Medicine)
- DBT (Program support)
- DST (FIST)
- UGC (Special assistance)
- Histone deacetylase inhibitors;
- sodium butyrate;
- growth arrest
Histone deacetylases inhibitors (HDIs) induce growth arrest and apoptosis in a variety of human cancer cells. Sodium butyrate (NaB), a histone deacetylase inhibitor, has been shown to cause a G1 cell cycle arrest by inducing p21WAF1/CIP1 in a p53-independent manner. In this report, we present evidence for activation of p53 pathway by NaB and its role in the NaB-mediated growth suppression. Addition of NaB increased the levels of p53 involving a p14ARF-dependent post-transcriptional mechanism. NaB induced p53 is functional as it activated p53-specific reporter, induced the level of p21WAF1/CIP1, inhibited cellular DNA synthesis and induced apoptosis. By using HPV 16 E6 stable transfectants as well as p53 null cancer cells, we show that NaB suppresses the growth of WT p53 containing cells more efficiently. NaB inhibited DNA synthesis to similar extent both in the presence and absence of p53. However, NaB treatment lead to a major G2/M arrest of cells in the presence of p53, while cells without wild-type p53 accumulated mainly in G1 phase of the cell cycle. Furthermore, apoptosis induction by NaB is greatly reduced in the absence of p53. These results suggest that p53 pathway mediates in part growth suppression by NaB and the p53 status may be an important determinant of chemosensitivity in HDI based cancer chemotherapy. © 2005 Wiley-Liss, Inc.