Increased expression of ICAM-3 is associated with radiation resistance in cervical cancer

Authors

  • Young Min Chung,

    1. Department of Internal Medicine and Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, South Korea
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    • The first 2 authors contributed equally to this paper.

  • Byoung-Gie Kim,

    1. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
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    • The first 2 authors contributed equally to this paper.

  • Chang-Soo Park,

    1. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
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  • Seung Jae Huh,

    1. Department of Radiation Oncology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
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  • Jhingook Kim,

    1. Department of Thoracic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
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  • Jong Kuk Park,

    1. Department of Internal Medicine and Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, South Korea
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  • Sun Mi Cho,

    1. Graduate School of Medicine, Korea University Cancer Institute, Seoul, South Korea
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  • Byung Soo Kim,

    1. Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea
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  • Jun Suk Kim,

    1. Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea
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  • Young Do Yoo,

    Corresponding author
    1. Graduate School of Medicine, Korea University Cancer Institute, Seoul, South Korea
    • Graduate School of Medicine, Korea University Cancer Institute, Korea University College of Medicine, Seoul 136-705, South Korea or Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea
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    • Fax: +82-2-926-4534.

  • Duk-Soo Bae

    Corresponding author
    1. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
    • Graduate School of Medicine, Korea University Cancer Institute, Korea University College of Medicine, Seoul 136-705, South Korea or Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea
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Abstract

To search for a marker that predicts the efficacy of radiation therapy in human cervical cancer, gene expression profiles between parental SiHa cervical cancer cells and radiation-resistant SiHa/R cells have been compared by the microarray technique. Microarray and Northern blot analyses demonstrated that the ICAM-3 expression was upregulated in SiHa/R cells. This increased expression of ICAM-3 in SiHa cells enhanced cell survival by about 34.3% after a 2 Gy dosage of radiation. In addition, SiHa/ICAM-3 cells showed a 2.45-fold higher level of FAK phosphorylation than that of the control cells. In tumor specimens, ICAM-3 staining was restricted to tumor stromal endothelial cells and lymphocytes. The overexpression of ICAM-3 was significantly more frequent in radiation-resistant cervical cancer specimens when compared with radiation-sensitive specimens (83.3% vs. 35.3%; p = 0.015). With these observations, we can suggest that an increased expression of ICAM-3 is associated with radiation resistance in cervical cancer cells and the expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy. © 2005 Wiley-Liss, Inc.

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