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Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers†‡

  1. Paul K. S. Chan1,2,§,*,
  2. Tak-Hong Cheung3,
  3. Ann O. Y. Tam2,
  4. Keith W. K. Lo3,
  5. So-Fan Yim3,
  6. May M. Y. Yu4,
  7. Ka-Fai To4,
  8. Yick-Fu Wong3,
  9. Jo L. K. Cheung2,
  10. Denise P. C. Chan2,
  11. Mamie Hui2,
  12. Margaret Ip2

Article first published online: 19 JUL 2005

DOI: 10.1002/ijc.21299

Issue

International Journal of Cancer

International Journal of Cancer

Volume 118, Issue 1, pages 243–245, 1 January 2006

Additional Information

How to Cite

Chan, P. K. S., Cheung, T.-H., Tam, A. O. Y., Lo, K. W. K., Yim, S.-F., Yu, M. M. Y., To, K.-F., Wong, Y.-F., Cheung, J. L. K., Chan, D. P. C., Hui, M. and Ip, M. (2006), Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers. International Journal of Cancer, 118: 243–245. doi: 10.1002/ijc.21299

Author Information

  1. 1

    School of Public Health, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China

  2. 2

    Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China

  3. 3

    Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China

  4. 4

    Department of Anatomical and Cellular Pathology, Faculty of Medicine, The Chinese University of Hong Kong,Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China

  1. §

    Fax: +852-2647-3227

*Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China

  1. †

    This study was presented in the 22nd International Papillomavirus Conference in Vancouver, 2–6 May 2005.

  2. ‡

    The authors do not have a commercial or other association that might pose a conflict of interest.

Publication History

  1. Issue published online: 26 OCT 2005
  2. Article first published online: 19 JUL 2005
  3. Manuscript Accepted: 18 APR 2005
  4. Manuscript Received: 31 JAN 2005

Funded by

  • Research Grants Council of the Hong Kong Special Administrative Region. Grant Number: CUHK4071/01M

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Keywords:

  • human papillomavirus;
  • detection;
  • genotype;
  • vaccine;
  • Chinese;
  • Hong Kong;
  • PCR

Abstract

Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type-specific sensitivity may result in misprioritization of vaccine candidates. © 2005 Wiley-Liss, Inc.

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