Cancer Cell Biology
Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells†
Article first published online: 14 NOV 2005
Copyright © 2005 Wiley-Liss, Inc.
International Journal of Cancer
Volume 118, Issue 8, pages 1922–1929, 15 April 2006
How to Cite
Liu, X., Sun, Y., Guo, J., Ma, H., Li, J., Dong, B., Jin, G., Zhang, J., Wu, J., Meng, L. and Shou, C. (2006), Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells. Int. J. Cancer, 118: 1922–1929. doi: 10.1002/ijc.21575
The present study provides the first demonstration that hiwi is highly expressed in gastric cancer cell lines and gastric cancer tissues. Notably, the expression levels are consistent with precancerous development. The suppression of hiwi by anti-sense or RNAi inhibited the growth of gastric cancer cells. These results suggest that hiwi may be involved in the development of gastric cancer and is a potential target for cancer therapy.
- Issue published online: 15 FEB 2006
- Article first published online: 14 NOV 2005
- Manuscript Accepted: 23 AUG 2005
- Manuscript Received: 27 MAY 2005
- National Natural Science Foundation of China. Grant Number: 30371611
- National High Technology Research and Development Program of China. Grant Number: 2002BA711A11A06
- Beijing Institute for Cancer Research
- gastric cancer;
Stem cell genetics research may be critical to our understanding of carcinogenesis, as both stem cells and cancer cells possess the ability to self-renew. Recent discoveries have indicated that the piwi family of genes plays an essential role in stem cell self-renewal in diverse organisms. The hiwi gene, the human homolog of the piwi family, participates in germ cell proliferation and its overexpression may cause the development of germ cell malignancy, but its expression and function in epithelial solid cancers have not been explored. In the present study, we investigated whether there was an association between hiwi expression and human gastric cancer and its potential mechanism. RT-PCR findings demonstrated that hiwi was expressed in different gastric cancer cell lines. To identify the HIWI protein in gastric cancer, we developed a specific monoclonal antibody against HIWI and immunohistochemistry was performed on various gastric tissues. We found that the expression ratio of hiwi in normal gastric tissues, atrophic gastritis, intestinal metaplasia and gastric cancers was 10% (5/50), 36% (18/50), 36% (18/50) and 76% (38/50), respectively, which was consistent with precancerous development. Notably, the expression pattern of hiwi in gastric cancer tissues was similar to that of Ki67, which was used as a marker of proliferation. Moreover, the suppression of hiwi by antisense or RNAi inhibited the growth of gastric cancer cells and induced cell cycle arrest in G2/M phase. These results suggest that hiwi may be involved in the development of gastric cancer and is a potential target for cancer therapy. © 2005 Wiley-Liss, Inc.