Identification of HLA class I dependent immunogenic peptides from clonotypic TCRβ expressed in cutaneous T-cell lymphoma

Authors

  • Jinyang Zeng,

    1. German Cancer Research Center (DKFZ), Skin Cancer Unit, Heidelberg, Germany
    2. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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    • Contributed equally to this artice: JZ and JM-B, SE and ED.

  • Jan Müller-Berghaus,

    1. German Cancer Research Center (DKFZ), Skin Cancer Unit, Heidelberg, Germany
    2. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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    • Contributed equally to this artice: JZ and JM-B, SE and ED.

  • Xuan Duc Nguyen,

    1. Institute for Transfusion Medicine and Immunology, Mannheim, Germany
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  • Harald Klüter,

    1. Institute for Transfusion Medicine and Immunology, Mannheim, Germany
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  • Hiltrud Schönhaber,

    1. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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  • Mingxia Song,

    1. German Cancer Research Center (DKFZ), Skin Cancer Unit, Heidelberg, Germany
    2. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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  • Nicole Schwinn,

    1. German Cancer Research Center (DKFZ), Skin Cancer Unit, Heidelberg, Germany
    2. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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  • Dirk Schadendorf,

    1. German Cancer Research Center (DKFZ), Skin Cancer Unit, Heidelberg, Germany
    2. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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  • Sergij Goerdt,

    1. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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  • Stefan Eichmüller,

    Corresponding author
    1. German Cancer Research Center (DKFZ), Skin Cancer Unit, Heidelberg, Germany
    2. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
    • German Cancer Research Center, D070, Box 101949, D-69009 Heidelberg, Germany
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    • Contributed equally to this artice: JZ and JM-B, SE and ED.

  • Edgar Dippel

    1. Department of Dermatology, University Hospital of Mannheim, Mannheim, Germany
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    • Contributed equally to this artice: JZ and JM-B, SE and ED.


Abstract

The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR β chain of the malignant T-cell population by the “reverse immunology” approach. Peptide-specific T-cell lines were generated against predicted epitopes and tested for the recognition of tumor cells and cells transfected with the full-length DNA coding for TCRV β chain. Two peptides derived from the clonotypic TCRVβ of a HLA-A2 positive patient could induce peptide-specific T cells from peripheral blood mononuclear cells of healthy donors and the patient as assessed by IFN-γ ELISpot assay. Furthermore, the reactive CTLs efficiently recognized autologous Sézary tumor cells, as well as HLA-A2 positive 293 cells transfected with recombinant plasmid expressing the corresponding TCRVβ29 protein. Similar results were obtained in a HLA-A3+ patient for TCRVβ7-Jβ2.7. In conclusion, our experiments show that the TCR β chain harbors epitopes suitable as targets for specific vaccination which might be a promising approach for the specific immunotherapy of cutaneous T-cell lymphoma patients. © 2006 Wiley-Liss, Inc.

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