Cancer-germline gene expression in pediatric solid tumors using quantitative real-time PCR
Article first published online: 3 OCT 2006
Copyright © 2006 Wiley-Liss, Inc.
International Journal of Cancer
Volume 120, Issue 1, pages 67–74, 1 January 2007
How to Cite
Jacobs, J. F.M., Brasseur, F., Hulsbergen-van de Kaa, C. A., van de Rakt, M. W.M.M., Figdor, C. G., Adema, G. J., Hoogerbrugge, P. M., Coulie, P. G. and de Vries, I. J. M. (2007), Cancer-germline gene expression in pediatric solid tumors using quantitative real-time PCR. Int. J. Cancer, 120: 67–74. doi: 10.1002/ijc.22118
- Issue published online: 30 OCT 2006
- Article first published online: 3 OCT 2006
- Manuscript Accepted: 27 APR 2006
- Manuscript Received: 17 MAR 2006
- The Quality of Life Gala
- Stichting Vrienden van het Kinderoncologisch Centrum Zuid-Oost Nederland
Cancer-germline genes (CGGs) code for immunogenic antigens that are present on various human tumors but not on normal tissues. The importance of CGGs in cancer immunotherapy has led to detailed studies of their expression in a range of human tumors. We measured the levels of expression of 12 CGGs in various pediatric solid tumors to identify targets for therapeutic cancer vaccines. Quantitative real-time PCR (qPCR) was used to measure the expression of 8 MAGE genes and of genes LAGE-2/NY-ESO-1 and GAGE-1, 2, 8 in 9 osteosarcomas, 10 neuroblastomas, 12 rhabdomyosarcomas and 18 Ewing's sarcomas. Nine tumors were also examined by immunohistochemistry with monoclonal antibodies specific for the MAGE-A1, MAGE-A4 and NY-ESO-1 proteins. All osteosarcoma and 80% of neuroblastoma samples expressed several CGGs at high levels. Six of 12 rhabdomyosarcomas and 11 of 18 Ewing's sarcomas expressed at least one CGG. Immunohistochemistry data correlated well with qPCR results and showed a homogeneous protein distribution pattern in most positive tumors. No correlation was found between the levels of CGG expression in the tumors and clinicopathological parameters of the patients. Pediatric solid tumors express several CGGs, which encode antigens that could be targeted in therapeutic vaccination trials. Several CGGs of the MAGE, GAGE and LAGE families are coexpressed in a large proportion of osteosarcoma and neuroblastoma samples. Some rhabdomyosarcomas express several of these genes at high levels. Ewing's sarcomas have an overall low CGG expression. © 2006 Wiley-Liss, Inc.