Early Detection and Diagnosis
αvβ3 Integrin-targeting of intraperitoneally growing tumors with a radiolabeled RGD peptide
Article first published online: 9 NOV 2006
Copyright © 2006 Wiley-Liss, Inc.
International Journal of Cancer
Volume 120, Issue 3, pages 605–610, 1 February 2007
How to Cite
Dijkgraaf, I., Kruijtzer, J. A.W., Frielink, C., Corstens, F. H.M., Oyen, W. J.G., Liskamp, R. M.J. and Boerman, O. C. (2007), αvβ3 Integrin-targeting of intraperitoneally growing tumors with a radiolabeled RGD peptide. Int. J. Cancer, 120: 605–610. doi: 10.1002/ijc.22297
- Issue published online: 29 NOV 2006
- Article first published online: 9 NOV 2006
- Manuscript Accepted: 3 AUG 2006
- Manuscript Received: 22 MAY 2006
- ovarian cancer;
- RGD peptide;
Ovarian cancer is the fourth most common cause of cancer deaths among females in the western world after cancer of the breast, colon and lung. The inability to control the disease within the peritoneal cavity is the major cause of treatment failure in patients with ovarian cancer. The majority of ovarian carcinomas express the αvβ3 integrin. Here we studied the tumor targeting potential of an 111In-labeled cyclic RGD peptide in athymic BALB/c mice with intraperitoneally (i.p.) growing NIH:OVCAR-3 human ovarian carcinoma tumors. The cyclic RGD peptide, c(RGDfK)E, was synthesized, conjugated with DOTA and radiolabeled with 111In. The targeting potential of 111In-DOTA-E-c(RGDfK) was studied in athymic mice with i.p. growing NIH:OVCAR-3 xenografts and the optimal dose of this compound was determined (0.01 μg up to 10 μg). The biodistribution at optimal peptide dose was determined at various time points (0.5 up to 72 hr). Furthermore, the therapeutic potential of 177Lu-DOTA-E-c(RGDfK) was studied in this model. Two hours after i.p. administration, 111In-DOTA-E-c(RGDfK) showed high and specific uptake in the i.p. growing tumors. Optimal uptake in the i.p. growing tumors was observed at a 0.03–0.1 μg dose range. Tumor uptake of 111In-DOTA-E-c(RGDfK) peaked 4 hr p.i. [(38.8 ± 2.7)% ID/g], gradually decreasing at later time points [(24.0 ± 4.1)% ID/g at 48 hr p.i.]. Intraperitoneal growth of OVCAR-3 could be significantly delayed by injecting 37 MBq 177Lu-labeled peptide i.p. Radiolabeled DOTA-E-c(RGDfK) is suitable for targeting of i.p. growing tumors and potentially can be used for peptide receptor radionuclide therapy of these tumors. © 2006 Wiley-Liss, Inc.