Functional genomics of calcium channels in human melanoma cells

Authors

  • Tamás Deli,

    1. Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
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    • The first four authors contributed equally.

  • Norbert Varga,

    1. Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary
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    • The first four authors contributed equally.

  • Attila Ádám,

    1. Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary
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    • The first four authors contributed equally.

  • István Kenessey,

    1. Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary
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    • The first four authors contributed equally.

  • Erzsébet Rásó,

    1. Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary
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  • László G. Puskás,

    1. Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
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  • József Tóvári,

    1. Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
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  • János Fodor,

    1. Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
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  • Mónika Fehér,

    1. Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
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  • Gyula P. Szigeti,

    1. Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
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  • László Csernoch,

    1. Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
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  • József Tímár

    Corresponding author
    1. Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary
    • Department of Tumor Progression, National Institute of Oncology, Ráth Gy. u. 7-9, Budapest H-1122, Hungary
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    • Fax: +36-1-224-8706


Abstract

Ca2+-signaling of human melanoma is in the focus of intensive research since the identification of the role of WNT-signaling in melanomagenesis. Genomic and functional studies pointed to the important role of various Ca2+ channels in melanoma, but these data were contradictory. In the present study we clearly demonstrate, in a number of different ways including microarray analysis, DNA sequencing and immunocytochemistry, that various human melanoma cell lines and melanoma tissues overexpress ryanodine receptor type 2 (RyR2) and express P2X7 channel proteins as compared to melanocytes. These channels, although retain some of their usual characteristics and pharmacological properties, display unique features in melanoma cells, including a functional interaction between the two molecules. Unlike P2X7, RyR2 does not function as a calcium channel. On the other hand, the P2X7 receptor has an antiapoptotic function in melanoma cells, since ATP-activation suppresses induced apoptosis, while knock down of the gene expression significantly enhances that. © 2007 Wiley-Liss, Inc.

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