Expression of S100A4 by a variety of cell types present in the tumor microenvironment of human breast cancer

Authors

  • Teresa Cabezón,

    1. Department of Proteomics in Cancer, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
    2. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
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  • Julio E. Celis,

    1. Department of Proteomics in Cancer, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
    2. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
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  • Inge Skibshøj,

    1. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
    2. Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
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  • Jörg Klingelhöfer,

    1. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
    2. Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
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  • Mariam Grigorian,

    1. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
    2. Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
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  • Pavel Gromov,

    1. Department of Proteomics in Cancer, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
    2. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
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  • Fritz Rank,

    1. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
    2. Department of Pathology, The Centre of Diagnostic Investigations, Rigshospitalet, Denmark
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  • June Helen Myklebust,

    1. Department of Immunology, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway
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  • Gunhild M. Mælandsmo,

    1. Department of Tumour Biology, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway
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  • Eugene Lukanidin,

    1. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
    2. Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
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  • Noona Ambartsumian

    Corresponding author
    1. Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Copenhagen, Denmark
    2. Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
    • Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, 4.3, 2100 Copenhagen, Denmark
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Abstract

The S100A4 protein, which is involved in the metastasis process, is a member of the S100 superfamily of Ca-binding proteins. Members of this family are multifunctional signaling proteins with dual extra and intracellular functions involved in the regulation of diverse cellular processes. Several studies have established a correlation between S100A4 protein expression and worse prognosis for patients with various malignancies including breast cancer. In this article, we have used specific antibodies in combination with immunohistochemistry (IHC) to identify the cell types that express S100A4 in human breast cancer biopsies obtained from high-risk patients. IHC analysis of 68 tumor biopsies showed that the protein is expressed preferentially by various cell types present in the tumor microenvironment (macrophages, fibroblasts, activated lymphocytes), rather than by the tumor cells themselves. Moreover, we show that the protein is externalized by the stroma cells to the fluid that bathes the tumor microenvironment, where it is found in several forms that most likely correspond to charge variants. Using a specific ELISA test, we detected a significant higher concentration of S100A4 in the tumor interstitial fluid (TIF) as compared to their corresponding normal counterparts (NIF). © 2007 Wiley-Liss, Inc.

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