Infectious Causes of Cancer
HPV prevalence, viral load and physical state of HPV-16 in cervical smears of patients with different grades of CIN†
Article first published online: 26 JUL 2007
Copyright © 2007 Wiley-Liss, Inc.
International Journal of Cancer
Volume 121, Issue 10, pages 2198–2204, 15 November 2007
How to Cite
Briolat, J., Dalstein, V., Saunier, M., Joseph, K., Caudroy, S., Prétet, J.-L., Birembaut, P. and Clavel, C. (2007), HPV prevalence, viral load and physical state of HPV-16 in cervical smears of patients with different grades of CIN. Int. J. Cancer, 121: 2198–2204. doi: 10.1002/ijc.22959
Original study on 363 women with cytology and a systematic histological control, addressing the interest of viral load, physical status of HPV-16 and single/multiple HPV infections on liquid-based cytology samples as diagnostic markers associated with different grades of histological lesions.
- Issue published online: 25 SEP 2007
- Article first published online: 26 JUL 2007
- Manuscript Accepted: 16 MAY 2007
- Manuscript Received: 26 DEC 2006
- Cancéropôle Grand-Est
- Région Champagne- Ardenne
- association “Un Euro contre le Cancer”
- Lion's Clubs of Soissons and Villers-Cotterets
- cervical cancer;
- viral load;
- viral DNA integration
Human papillomavirus (HPV) infection is the most important event in malignant transformation of human cervical epithelium. We analysed in cervical smears, HPV genotypes with a focus on single/multiple infections, then characteristics of HPV-16 infections (presence of other genotypes, viral load and physical state) according to the grade of histological lesions. The purpose of this study was to know if these parameters could allow to differentiate histological diagnoses. DNA was extracted from 363 cervical samples corresponding to 24 cases without lesion, 96 CIN1, 92 CIN2, 144 CIN3 and 7 cancers. Our results show that HPV-16 was predominant and its prevalence increased with the severity of lesions (CIN1: 27.1%; CIN3: 65.3%). In addition, we showed that the frequency of single infections, as compared with multiple infections, increased with the severity of the lesion (CIN1: 25.0%; CIN3: 54.8%). Among HPV-16 positive samples (n = 170), we found that viral load, determined on cervical samples by real-time PCR, did not vary significantly according to the different CIN grades. Concerning HPV-16 integration, the mixed and integrated HPV-16 forms, already present in women with normal histology, increased to the benefit of pure episomal forms with the severity of lesions (normal cervix: 28.6%; CIN3: 73.8%). Thus, our data raise the question of the viral load as a valuable clinical parameter to discriminate between lesion grades. Moreover, we emphasize integration as an early event in cervical carcinogenesis, increasing with the severity of lesions. Finally, this study underlines the importance of single versus multiple infections linked to the severity of CIN. © 2007 Wiley-Liss, Inc.