Fax: +49-89-324-266-199.
Cancer Therapy
You have full text access to this OnlineOpen article
Bi20 (fBTA05), a novel trifunctional bispecific antibody (anti-CD20 × anti-CD3), mediates efficient killing of B-cell lymphoma cells even with very low CD20 expression levels
Article first published online: 10 JUN 2008
DOI: 10.1002/ijc.23626
Copyright © 2008 Wiley-Liss, Inc.
Additional Information
How to Cite
Stanglmaier, M., Faltin, M., Ruf, P., Bodenhausen, A., Schröder, P. and Lindhofer, H. (2008), Bi20 (fBTA05), a novel trifunctional bispecific antibody (anti-CD20 × anti-CD3), mediates efficient killing of B-cell lymphoma cells even with very low CD20 expression levels. Int. J. Cancer, 123: 1181–1189. doi: 10.1002/ijc.23626
Publication History
- Issue published online: 17 JUN 2008
- Article first published online: 10 JUN 2008
- Manuscript Accepted: 22 FEB 2008
- Manuscript Received: 26 JUN 2007
References
- 1
- 2,,,,,,,,,,,, et al. SEER Cancer Statistics Review 1975–2003, National Cancer Institute. Bethesda, MD, 2006. http://seer.cancer.gov.
- 3,,. Rituximab for the treatment of diffuse large B-cell lymphomas. Expert Rev Anticancer Ther 2006; 6: 1175–86.
- 4Monoclonal antibody therapy for B-cell malignancies. Semin Oncol 2006; 33: S2–14.
- 5. Monoclonal antibody as therapy for malignant lymphomas. C R Biol 2006; 329: 241–54.
- 6. Rituximab (monoclonal anti-CD20 antibody): mechanisms of action and resistance. Oncogene 2003; 22: 7359–68.
- 7,. Novel therapies for chronic lymphocytic leukemia. Blood Rev 2004; 18: 137–48.
- 8,,,,,,,,,,,, et al. Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells. Nat Med 2002; 8: 801–7.
- 9,,. Bispecific antibodies for polyclonal T-cell engagement. Curr Opin Mol Ther 2003; 5: 413–9.
- 10,. Bispecific antibodies targeting cancer cells. Biochem Soc Trans 2002; 30: 507–11.
- 11,,,,,,,. Locoregional treatment of low-grade B-cell lymphoma with CD3xCD19 bispecific antibodies and CD28 costimulation. I. Clinical phase I evaluation Int J Cancer 2001; 91: 508–15.
- 12,,,,. Locoregional treatment of low-grade B-cell lymphoma with CD3xCD19 bispecific antibodies and CD28 costimulation. II. Assessment of cellular immune responses. Int J Cancer 2001; 91: 516–22.Direct Link:
- 13. Performance of CD3xCD19 bispecific monoclonal antibodies in B cell malignancy. Leuk Lymphoma 1995; 19: 381–93.
- 14,,,,,,,. The Fc-region of a new class of intact bispecific antibody mediates activation of accessory cells and NK cells and induces direct phagocytosis of tumour cells. Br J Cancer 2000; 83: 261–6.
- 15,,,,,,. Simultaneous activation of T cells and accessory cells by a new class of intact bispecific antibody results in efficient tumor cell killing. J Immunol 1999; 163: 1246–52.
- 16,,,,. Bispecific antibodies target operationally tumor-specific antigens in two leukemia relapse models. Blood 1996; 88: 4651–8.
- 17,. Induction of a long-lasting antitumor immunity by a trifunctional bispecific antibody. Blood 2001; 98: 2526–34.
- 18,,,,,,,,. Immunotherapy of malignant ascites with trifunctional antibodies. Int J Cancer 2005; 117: 435–43.Direct Link:
- 19,,,. Lysis of prostate carcinoma cells by trifunctional bispecific antibodies (alpha EpCAM × alpha CD3). J Histochem Cytochem 2001; 49: 911–7.
- 20,,,,,,,,,,,. Phase I trial of the trifunctional anti-HER2 × anti-CD3 antibody ertumaxomab in metastatic breast cancer. Clin Cancer Res 2006; 12: 3085–91.
- 21,. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol Today 1994; 15: 450–4.
- 22,,,. Mice carrying a CD20 gene disruption. Immunogenetics 1998; 48: 125–32.
- 23,,,,,. A unique cell surface antigen identifying lymphoid malignancies of B cell origin. J Clin Invest 1981; 67: 134–40.
- 24,,,,,,,. Diagnostic significance of CD20 and FMC7 expression in B-cell disorders. Am J Clin Pathol 2003; 120: 754–9.
- 25,,,,,. Expression of human B cell-associated antigens on leukemias and lymphomas: a model of human B cell differentiation. Blood 1984; 63: 1424–33.
- 26,,,. Retention of B-cell-specific monoclonal antibodies by human lymphoma cells. Blood 1994; 83: 1390–7.
- 27,,,. Preferential species-restricted heavy/light chain pairing in rat/mouse quadromas. Implications for a single-step purification of bispecific antibodies. J Immunol 1995; 155: 219–25.
- 28,,,,,. Two new trifunctional antibodies for the therapy of human malignant melanoma. Int J Cancer 2004; 108: 725–32.Direct Link:
- 29,,,,,,,. Treatment of human B cell lymphoma xenografts with a CD3 × CD19 diabody and T cells. J Immunol 2000; 165: 888–95.
- 30,,,,,. Cytokine-release syndrome in patients with B-cell chronic lymphocytic leukemia and high lymphocyte counts after treatment with an anti-CD20 monoclonal antibody (rituximab. IDEC-C2B8). Blood 1999; 94: 2217–24.
- 31,,. Impairments in immune cell function in B cell chronic lymphocytic leukemia. Semin Oncol 1998; 25: 27–33.
- 32
- 33,. Bispecific monoclonal antibody therapy of B-cell malignancy. Leuk Lymphoma 1995; 16: 199–207.
- 34
- 35,,,,,. Higher levels of surface CD20 expression on circulating lymphocytes compared with bone marrow and lymph nodes in B-cell chronic lymphocytic leukemia. Am J Clin Pathol 2001; 116: 437–43.
- 36,,,. Modulating apoptosis pathways in low-grade B-cell malignancies using biological response modifiers. Semin Oncol 2002; 29: 10–24.
- 37,,,,,,,. Characterization of BIS20x3, a bi-specific antibody activating and retargeting T-cells to CD20-positive B-cells. Br J Cancer 2001; 84: 1115–21.
- 38,,,,,,,,,,,. Efficient inhibition of human B-cell lymphoma xenografts with an anti-CD20 × anti-CD3 bispecific diabody. Cancer Lett 2002; 177: 29–39.
- 39,,,. Bispecific CD3 × CD19 diabody for T cell-mediated lysis of malignant human B cells. Int J Cancer 1998; 77: 763–72.Direct Link:
- 40,,. Rituximab in B-cell chronic lymphocytic leukemia. Semin Oncol 2003; 30: 483–92.
- 41,,,,. IDEC-C2B8 anti-CD20 (rituximab) immunotherapy in patients with low-grade non-Hodgkin's lymphoma and lymphoproliferative disorders: evaluation of response on 48 patients. Eur J Haematol 1999; 62: 76–82.Direct Link:
- 42,,,,,. CD3xCD19 bispecific antibodies and CD28 bivalent antibodies enhance T-cell reactivity against autologous leukemic cells in pediatric B-ALL bone marrow. Int J Cancer 1999; 80: 715–22.Direct Link:
- 43,,,,. CD3 × CD19 bispecific antibodies and CD28 costimulation for locoregional treatment of low-malignancy non-Hodgkin's lymphoma. Cancer Immunol Immunother 1997; 45: 198–202.
- 44,,. Issues in T-helper 1 development–resolved and unresolved. Immunol Rev 2004; 202: 157–74.Direct Link:
- 45,. T and B cells in B-chronic lymphocytic leukaemia: Faust, Mephistopheles and the pact with the Devil. Cancer Immunol Immunother 2006; 55: 210–20.
- 46,,,,,. Zeta chain and CD28 are poorly expressed on T lymphocytes from chronic lymphocytic leukemia. Leukemia 1996; 10: 494–7.
- 47,,,,,,,,,. Extremely potent, rapid and costimulation-independent cytotoxic T-cell response against lymphoma cells catalyzed by a single-chain bispecific antibody. Int J Cancer 2002; 100: 690–7.Direct Link:
- 48,,,,,,,,,,,. Efficient elimination of chronic lymphocytic leukaemia B cells by autologous T cells with a bispecific anti-CD19/anti-CD3 single-chain antibody construct. Leukemia 2003; 17: 900–9.
- 49,,,,,,,. Synergistic antitumor effect of bispecific CD19 × CD3 and CD19 × CD16 diabodies in a preclinical model of non-Hodgkin's lymphoma. J Immunol 2002; 169: 137–44.
- 50,,,,,,. Adoptive immunotherapy of B-cell malignancies with a trifunctinal, bispecific antibody (anti-CD3 × anti-CD20) and allogeneic donor lymphocyte transfusion. Haematologica 2006; 91: 102a.

1097-0215/asset/olbannerleft.jpg?v=1&s=45719cd7de57873027993264fcc568b335a8cd56)
1097-0215/asset/olbannerright.jpg?v=1&s=5e0fba63c1309b3036eb9215a0e1e83dd02efd19)
