Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity

Authors

  • Shohei Domae,

    1. Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
    2. Department of Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Yoichi Nakamura,

    1. Department of Internal Medicine, Nagasaki University School of Medicine, Dentistry and Pharmaceutical Science, 1-7-1 Sakamoto-machi, Nagasaki, Japan
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  • Yurika Nakamura,

    1. Department of Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, Japan
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  • Akiko Uenaka,

    1. Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Hisashi Wada,

    1. Department of Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, Japan
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  • Masao Nakata,

    1. Department of Thoracic Surgery, Kawasaki Medical School, 577 Matsushima, Kurashiki, Japan
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  • Mikio Oka,

    1. Department of Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki, Japan
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  • Koji Kishimoto,

    1. Department of Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Goichi Tsukamoto,

    1. Department of Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Yasuto Yoshihama,

    1. Department of Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Junji Matsuoka,

    1. Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Akira Gochi,

    1. Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Shigeru Kohno,

    1. Department of Internal Medicine, Nagasaki University School of Medicine, Dentistry and Pharmaceutical Science, 1-7-1 Sakamoto-machi, Nagasaki, Japan
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  • Takashi Saika,

    1. Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Akira Sasaki,

    1. Department of Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Eiichi Nakayama,

    1. Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1 Shikata-cho, Okayama, Japan
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  • Toshiro Ono

    Corresponding author
    1. Department of Radiation Research, Advanced Science Research Center, Okayama University, 2-5-1 Shikata-cho, Okayama, Japan
    • Department of Radiation Research, Advanced Science Research Center, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
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    • Fax: +81-86-221-2270


Abstract

Cancer/testis (CT) antigens are expressed in normal germ line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis-specific expression profiles in the Unigene database, including coiled-coil domain containing 62 (CCDC62). RT-PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62-2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62-2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62-2 was confirmed by western blot. CCDC62-2 is a CT antigen that is immunogenic in cancer patients. © 2008 Wiley-Liss, Inc.

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