Systemic inflammation increases cancer cell adhesion to hepatic sinusoids by neutrophil mediated mechanisms

Authors

  • Braedon McDonald,

    1. LD McLean Surgical Research Laboratories, Department of Surgery, McGill University, Montreal, Quebec, Canada
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    • The first two authors contributed equally to this work.

  • Jonathan Spicer,

    1. LD McLean Surgical Research Laboratories, Department of Surgery, McGill University, Montreal, Quebec, Canada
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    • The first two authors contributed equally to this work.

  • Betty Giannais,

    1. LD McLean Surgical Research Laboratories, Department of Surgery, McGill University, Montreal, Quebec, Canada
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  • Lucia Fallavollita,

    1. LD McLean Surgical Research Laboratories, Department of Surgery, McGill University, Montreal, Quebec, Canada
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  • Pnina Brodt,

    1. LD McLean Surgical Research Laboratories, Department of Surgery, McGill University, Montreal, Quebec, Canada
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  • Lorenzo E. Ferri

    Corresponding author
    1. LD McLean Surgical Research Laboratories, Department of Surgery, McGill University, Montreal, Quebec, Canada
    2. Division of Thoracic Surgery, Department of Surgery, McGill University, Montreal, Quebec, Canada
    • Division of Thoracic Surgery, McGill University, The Montreal General Hospital, Room L9-112, 1650 Cedar Ave, Montreal, Quebec, Canada H3G 1A4
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Abstract

Interactions between endothelial selectins and selectin ligands expressed on tumor cells have been implicated in the binding of circulating metastatic cancer cells to the vascular endothelium during extravasation. Moreover, there is mounting evidence that inflammatory environments can accelerate the progression of metastasis by neutrophil mediated mechanisms. In this study, a physiologically relevant in vivo model of early metastasis coupled with intravital microscopy was used to visualize the trafficking of tumor cells within the liver vasculature in real time. Using GFP-labeled Lewis lung carcinoma subline H-59 cells, we show here that disrupting the interactions between endothelial selectins and tumor cell selectin ligands diminished tumor cell recruitment to the liver. Furthermore, systemic inflammation induced by intravenous injection of lipopolysaccharide significantly enhanced the metastatic potential of these lung carcinoma cells by increasing their propensity to adhere to the liver sinusoidal endothelium. Confocal microscopy revealed frequent colocalization of cancer cells with neutrophils and neutrophil depletion in vivo significantly attenuated the lipopolysaccharide-induced increase in H-59 cell adhesion. Although direct selectin–selectin ligand interactions contributed significantly to tumor cell adhesion to sinusoidal endothelial cells, we show here that in addition, interactions between adherent neutrophils within the inflamed sinusoids and circulating tumor cells may further increase tumor cell arrest in the liver. © 2009 UICC

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