Downregulation of p21-activated kinase-1 inhibits the growth of gastric cancer cells involving cyclin B1

Authors

  • Funan Liu,

    1. Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning, People's Republic of China
    2. Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China
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  • Xiaodong Li,

    1. Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning, People's Republic of China
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  • Chunyu Wang,

    1. Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning, People's Republic of China
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  • Xinze Cai,

    1. Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning, People's Republic of China
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  • Zhongmin Du,

    1. Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China
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  • Huimian Xu,

    Corresponding author
    1. Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China
    • Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, People's Republic of China
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  • Feng Li

    Corresponding author
    1. Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning, People's Republic of China
    • Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning 110001, People's Republic of China
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    • Fax: +86-24-23261056.

Errata

This article is corrected by:

  1. Errata: Errata Volume 126, Issue 3, 799, Article first published online: 24 November 2009

Abstract

p21-activated kinase 1 (Pak1), a serine/threonine kinase, has been implicated in cytoskeletal remodelling, cell motility, apoptosis and transformation. However, the role of Pak1 in gastric cancer remains unclear. In this study, we detected Pak1 expression in gastric cancer tissues from 40 patients by western blot. Overexpression of Pak1 was associated with progression, metastasis and prognosis of gastric cancer. In addition, we found that knockdown of Pak1 expression significantly inhibited anchorage-dependent and anchorage-independent growth in gastric cancer cells, and markedly inhibited gastric cancer cell xenograft tumor growth. In further study, data showed that Pak1 could regulate the expression of cyclin B1 at the mRNA and protein levels, and impact the subcellular distribution and the promoter activity of cyclin B1. Results from deletion and mutant analysis supplied a new NF-κB binding sites at position -321 of cyclin B1 promoter, and indicated that Pak1 regulated the transcription of cyclin B1 in gastric cancer through NF-κB. In conclusion, Pak1 may be a potential prognostic marker and therapeutic target in gastric cancer. © 2009 UICC

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