Early Detections and Diagnosis

You have full text access to this OnlineOpen article

Diagnostic and prognostic implications of microRNA profiling in prostate carcinoma

  1. Annika Schaefer1,2,3,†,
  2. Monika Jung2,†,
  3. Hans-Joachim Mollenkopf4,
  4. Ina Wagner4,
  5. Carsten Stephan2,
  6. Florian Jentzmik2,
  7. Kurt Miller2,
  8. Michael Lein2,3,
  9. Glen Kristiansen5,†,
  10. Klaus Jung2,3,†,‡,*

Article first published online: 12 AUG 2009

DOI: 10.1002/ijc.24827

Issue

International Journal of Cancer

International Journal of Cancer

Volume 126, Issue 5, pages 1166–1176, 1 March 2010

Additional Information

How to Cite

Schaefer, A., Jung, M., Mollenkopf, H.-J., Wagner, I., Stephan, C., Jentzmik, F., Miller, K., Lein, M., Kristiansen, G. and Jung, K. (2010), Diagnostic and prognostic implications of microRNA profiling in prostate carcinoma. Int. J. Cancer, 126: 1166–1176. doi: 10.1002/ijc.24827

Author Information

  1. 1

    Department of Biology, Chemistry and Pharmacy, Free University, Berlin, Germany

  2. 2

    Department of Urology, University Hospital Charité, Berlin, Germany

  3. 3

    Berlin Institute for Urologic Research, Berlin, Germany

  4. 4

    Max Planck Institute for Infection Biology, Berlin, Germany

  5. 5

    Department of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland

  1. †

    Annika Schaefer, Monika Jung, Glen Kristiansen and Klaus Jung contributed equally to this work

  2. ‡

    Fax: +4930450515904

*Department of Urology, University Hospital Charité, Schumannstr. 20/21, Berlin 10117, Germany

Publication History

  1. Issue published online: 27 DEC 2009
  2. Article first published online: 12 AUG 2009
  3. Accepted manuscript online: 12 AUG 2009 12:00AM EST
  4. Manuscript Accepted: 4 AUG 2009
  5. Manuscript Received: 24 APR 2009

Funded by

  • Urologic Research Foundation Berlin, SONNENFELD Foundation

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (473K)

Keywords:

  • prostate cancer;
  • microRNA profiling;
  • diagnostic and prognostic biomarkers;
  • hsa-miR-96

Abstract

This study aimed to investigate the microRNA (miRNA) profile in prostate carcinoma tissue by microarray analysis and RT-qPCR, to clarify associations of miRNA expression with clinicopathologic data and to evaluate the potential of miRNAs as diagnostic and prognostic markers. Matched tumor and adjacent normal tissues were obtained from 76 radical prostatectomy specimens. Twenty-four tissue pairs were analyzed using human miRNA microarrays for 470 human miRNAs. Differentially expressed miRNAs were validated by TaqMan RT-qPCR using all 76 tissue pairs. The diagnostic potential of miRNAs was calculated by receiver operating characteristics analyses. The prognostic value was assessed in terms of biochemical recurrence using Kaplan–Meier and Cox regression analyses. Fifteen differentially expressed miRNAs were identified with concordant fold-changes by microarray and RT-qPCR analyses. Ten microRNAs (hsa-miR-16, hsa-miR-31, hsa-miR-125b, hsa-miR-145, hsa-miR-149, hsa-miR-181b, hsa-miR-184, hsa-miR-205, hsa-miR-221, hsa-miR-222) were downregulated and 5 miRNAs (hsa-miR-96, hsa-miR-182, hsa-miR-182*, hsa-miR-183, hsa-375) were upregulated. Expression of 5 miRNAs correlated with Gleason score or pathological tumor stage. Already 2 microRNAs classified up to 84% of malignant and nonmalignant samples correctly. Expression of hsa-miR-96 was associated with cancer recurrence after radical prostatectomy and that prognostic information was confirmed by an independent tumor sample set from 79 patients. That was shown with hsa-miR-96 and the Gleason score as final variables in the Cox models build in the 2 patient sets investigated. Thus, differential miRNAs in prostate cancer are useful diagnostic and prognostic indicators. This study provides a solid basis for further functional analyses of miRNAs in prostate cancer.

View Full Article with Supporting Information (HTML) Get PDF (473K)