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Additional Supporting Information may be found in the online version of this article.

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IJC_24866_sm_supfig1.tif2632KSupplementary Figure S1. BEX2 and BEX1 expression using Real-Time PCR in cell lines. (a) BEX2 expression folds in breast cancer cell lines relative to that of MCF-7 using RT-PCR. Error Bars: ± 2SEM. (b) BEX1 expression was assessed in breast cancer cell lines MCF-7, MDA-MB-231, T-47D, BT-474, Sum190, MDA-MB-361, SK-BR-3, and MDA-MB-453; prostate cancer cell line LNCap; and glioma cell lines U251MG and U87MG. Expression values are shown as: (40-CT) and a value of 0 means no detected expression.
IJC_24866_sm_supfig2.tif2094KSupplementary Figure S2. Real Time-PCR showing BEX2-knock down in breast cancer cell lines. BEX2-knock down efficiencies are shown for siRNA duplex 1/2 (Dup1/2), duplex 3/4 (Dup3/4), and duplex 5/6 (Dup 5/6) in MCF-7, MDA-MB-231, and T-47D cell lines. Expression of BEX2 after knock-down was assessed relative to non-targeting siRNA control and fold changes are shown for each duplex in each cell line.
IJC_24866_sm_supfig3.tif319KSupplementary Figure S3. The effect of BEX2 on ceramide pro-apoptotic response. (a) Percentage of apoptosis in MCF-7 cells after BEX2-knock down (KD) and treatment with ceramide. Ceramide treatment was carried out at 3 μM, 7 μM, and 10 μM concentrations overnight and apoptosis was measured using Hoechst assay. Non-targeting siRNA was used as a control. (b) Percentage of apoptosis in MDA-MB-231 cells as described in A. (c) Percentage of apoptosis in T-47D cells as described in A. (d) Hoechst staining to show the apoptotic cells in MDA-MB-231 cells after BEX2-KD and ceramide treatment at 3 μM. All error bars indicate: ± 2SEM.
IJC_24866_sm_supfig4.tif208KSupplementary Figure S4. BEX2-Knock Down and doxorubicin treatment. Histograms show the percentage of apoptosis using Annexin V-FITC assay inMDA-MB-231 cells. Cells were transfected with the control siRNA (a) or BEX2-siRNA (b) followed by doxorubicin treatment at 200 nM overnight.
IJC_24866_sm_supfig5.tif1426KSupplementary Figure S5. The effect of BEX2 down-regulation on p21. CDKN1A transcriptional change after BEX2 Knock-Down. RT-PCR was carried out in MCF-7 and MDA-MB-231 cells after transfections with either control-siRNA (control) or BEX2-siRNA. CDKN1A expression fold to control group is demonstrated for both cell lines. *, p < 0.03 is for the difference between knock-down and control groups in MDA-MB-231 cells. Error Bars: ± 2SEM.
IJC_24866_sm_supTable-1.doc28KSupplementary Table 1. Percentage of p65 nuclear staining.

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