Multiple serological biomarkers for colorectal cancer detection

Authors

  • Chung-Chuan Chan,

    1. Department of Gastroenterology, Hsinchu Cathay General Hospital, 678 Section 2, Junghua Road, Hsinchu, Taiwan
    2. College of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei City, Taiwan
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    • The first three authors contributed equally to this work

  • Chung-Wei Fan,

    1. Department of Colorectal Surgery, Chang Gung Memorial Hospital, 222 Maijin Road, Keelung, Taiwan
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    • The first three authors contributed equally to this work

  • Yung-Bin Kuo,

    1. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan, Taiwan
    2. College of Biological Science and Technology, National Chiao Tung University, 75 Bo-Ai Street, Hsinchu, Taiwan
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    • The first three authors contributed equally to this work

  • Yu-Han Chen,

    1. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan, Taiwan
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  • Pi-Yueh Chang,

    1. Department of Clinical Pathology, Chang Gung Memorial Hospital, 5 Fu-Hsing Street, Kwei-Shan, Taoyuan, Taiwan
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  • Kuei-Tien Chen,

    1. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan, Taiwan
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  • Ray-Ping Hung,

    1. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan, Taiwan
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  • Err-Cheng Chan

    Corresponding author
    1. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan, Taiwan
    • Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-shan, Taoyuan, Taiwan, Republic of China
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    • Tel.: +886-3-2118800 (ext. 5220), Fax: +886-3-2118741


Abstract

The aim of this study was to initiate a survey of human autoantibody responses to a panel of select colorectal tumor-associated antigens identified by previous serological analysis of a cDNA expression library and to subsequently identify multiple serological biomarkers for the detection of colorectal cancer. For screening of autoantibodies against colorectal tumor-associated antigens, sera from 94 colorectal cancer patients and 54 normal controls were analyzed by enzyme-linked immunosorbent assay using recombinant rCCCAP, rHDAC5, rP53, rNMDAR and rNY-CO-16 proteins as coating antigens. Seropositivity among colorectal cancer patients to the 5 individual coating antigens varied from 18.1% to 35.1%. Seropositivity to any of the 5 coating antigens was 58.5% and combining this analysis with evaluation of serum carcinoembryonic antigen (≥5 ng/ml) significantly increased the seropositivity to 77.6%. Seropositivity of early-stage (Dukes' Stages A and B) colorectal cancer patients to CEA was 21.9%, and seropositivity to any of the 5 colorectal cancer-associated antigens was 53.7%, and the combination of these 2 measurements resulted in a higher diagnostic capacity (65.9%) than either marker alone. In conclusion, these results collectively indicated that combined detection of serum autoantibody profiles against our panel of colorectal tumor-associated antigens and the analysis of carcinoembryonic antigen provides a promising diagnostic biomarker for colorectal cancer, particularly among early-stage patients.

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