Fast Track

You have full text access to this OnlineOpen article

Prevention of cigarette smoke–induced lung tumors in mice by budesonide, phenethyl isothiocyanate, and N-acetylcysteine

  1. Roumen Balansky1,2,
  2. Gancho Ganchev1,
  3. Marietta Iltcheva1,
  4. Vernon E. Steele3,
  5. Silvio De Flora2,†,*

Article first published online: 8 OCT 2009

DOI: 10.1002/ijc.24942

Issue

International Journal of Cancer

International Journal of Cancer

Volume 126, Issue 5, pages 1047–1054, 1 March 2010

Additional Information

How to Cite

Balansky, R., Ganchev, G., Iltcheva, M., Steele, V. E. and De Flora, S. (2010), Prevention of cigarette smoke–induced lung tumors in mice by budesonide, phenethyl isothiocyanate, and N-acetylcysteine. Int. J. Cancer, 126: 1047–1054. doi: 10.1002/ijc.24942

Author Information

  1. 1

    National Center of Oncology, Sofia, Bulgaria

  2. 2

    Department of Health Sciences, University of Genoa, Genoa, Italy

  3. 3

    National Cancer Institute, Rockville, MD

  1. †

    Tel: +39-010-353-8500

*Department of Health Sciences, University of Genoa, Via A. Pastore 1, I-16132 Genoa, Italy

Publication History

  1. Issue published online: 27 DEC 2009
  2. Article first published online: 8 OCT 2009
  3. Accepted manuscript online: 8 OCT 2009 12:00AM EST
  4. Manuscript Accepted: 15 SEP 2009
  5. Manuscript Received: 4 AUG 2009

Funded by

  • The Bulgarian Ministry of Education and Science
  • The U.S. National Cancer Institute. Grant Number: N01-CN53301

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (183K)

References

  • 1
    International Agency for Research on Cancer. Tobacco smoke and involuntary smoking. IARC monographs on the evaluation of the carcinogenic risks to humans, vol. 83. Lyon: IARC, 2004.
  • 2
    Blot WJ, Fraumeni JF Jr. Cancers of the lung and pleura. In: SchottenfeldD, FraumeniJFJr. Cancer Epidemiology and Prevention. New York: Oxford University Press, 1996: 637–65.
  • 3
    Winterhalder RC, Hirsch FR, Kotantoulas GK, Franklin WA, Bunn PA Jr. Chemoprevention of lung cancer—from biology to clinical reality. Ann Oncol 2004; 15: 185–96.
  • 4
    American Cancer Society. Tobacco use. In: Cancer Facts & Figures 2001. Atalanta, 2001: 4–5.
  • 5
    De Flora S, Quaglia A, Bennicelli C, Vercelli M. The epidemiological revolution of the 20th Century. FASEB J 2005; 19: 892–7.
  • 6
    Hirsch FR, Lippman SM. Advances in the biology of lung cancer chemoprevention. J Clin Oncol 2005; 23: 3186–97.
  • 7
    van Zandwijk N. Chemoprevention in lung carcinogenesis—an overview. Eur J Cancer 2005; 41: 1990–2002.
  • 8
    Omenn GS. Chemoprevention of lung cancers: lessons from CARET, the beta-carotene and retinol efficacy trial, and prospects for the future. Eur J Cancer Prev 2007; 16: 184–91.
  • 9
    Hecht SS. Cigarette smoking and lung cancer: chemical mechanisms and approaches to prevention. Lancet Oncol 2002; 3: 461–9.
  • 10
    Witschi H. The complexities of an apparently simple lung tumor model: The A/J mouse. Exp Toxicol Pathol 2005; 57 Suppl 1: 171–81.
  • 11
    Mertens VE. Zigarettenrauch, eine Ursache des Lungenkrebses. Z Krebsforsch 1930; 32: 82.
  • 12
    Lorenz E, Stewart HL, Daniel JH, Warren S. The effects of breathing tobacco smoke on strain A mice. Cancer Res 1943; 3: 123.
  • 13
    Hecht SS. Carcinogenicity studies of inhaled cigarette smoke in laboratory animals: old and new. Carcinogenesis 2005; 26: 1488–92.
  • 14
    Coggins CR. A minireview of chronic animal inhalation studies with mainstream cigarette smoke. Inhal Toxicol 2002; 14: 991–1002.
  • 15
    Witschi H. A/J mouse as a model for lung tumorigenesis caused by tobacco smoke: strengths and weaknesses. Exp Lung Res 2005; 31: 3–18.
  • 16
    De Flora S, D'Agostini F, Balansky R, Camoirano A, Bennicelli C, Bagnasco M, Cartiglia C, Tampa E, Longobardi M, Lubet RA, Izzotti A. Modulation of cigarette smoke-related end-points in mutagenesis and carcinogenesis. Mutat Res 2003; 523–524: 237–52.
  • 17
    Mauderly JL, Gigliotti AP, Barr EB, Bechtold WE, Belinsky SA, Hahn FF, Hobbs CA, March TH, Seilkop SK, Finch GL. Chronic inhalation exposure to mainstream cigarette smoke increases lung and nasal tumor incidence in rats. Toxicol Sci 2004; 81: 280–92.
  • 18
    Hutt JA, Vuillemenot BR, Barr EB, Grimes MJ, Hahn FF, Hobbs CH, March TH, Gigliotti AP, Seilkop SK, Finc GL, Mauderly JL, Belinsky SA. Life-span inhalation exposure to mainstream cigarette smoke induces lung cancer in B6C3F1 mice through genetic and epigenetic pathways. Carcinogenesis 2005; 26: 1999–2009.
  • 19
    Balansky RM, Ganchev G, Iltcheva M, Steele VE, D'Agostini F, De Flora S. Potent carcinogenicity of cigarette smoke in mice exposed early in life. Carcinogenesis 2007; 28: 2236–43.
  • 20
    Wattenberg LW, Wiedmann TS, Estensen RD, Zimmerman CL, Galbraith AR, Steele VE, Kelloff GJ. Chemoprevention of pulmonary carcinogenesis by brief exposures to aerosolized budesonide or beclomethasone dipropionate and by the combination of aerosolized budesonide and dietary myo-inositol. Carcinogenesis 2000; 21: 179–82.
  • 21
    De Flora S, Izzotti A, D'Agostini F, Balansky RM. Mechanisms of N–acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 2001; 22: 999–1013.
  • 22
    Balansky R, Blagoeva PM, Mircheva ZI. Investigation of the mutagenic activity of tobacco smoke. Mutat Res 1987; 188: 13–9.
  • 23
    Balansky R, D'Agostini F, Zanacchi P, De Flora S. Protection by N-acetylcysteine of the histopathological and cytogenetical damage produced by exposure of rats to cigarette smoke. Cancer Lett 1992; 64: 123–31.
  • 24
    Balansky R, D'Agostini F, De Flora S. Induction, persistence and modulation of cytogenetic alterations in cells of smoke-exposed mice. Carcinogenesis 1999; 20: 1491–7.
  • 25
    Bagnasco M, Bennicelli C, Camoirano A, Balansky R, De Flora S. Metabolic alterations produced by cigarette smoke in rat lung and liver, and their modulation by oral N-acetylcysteine. Mutagenesis 1992; 7: 295–301.
  • 26
    Balansky R, Izzotti A, Scatolini L, D'Agostini F, De Flora S. Induction by carcinogens and chemoprevention by N-acetylcysteine of adducts to mitochondrial DNA in rat organs. Cancer Res 1996; 56: 1642–7.
  • 27
    D'Agostini F, Balansky R, Izzotti A, Lubet RA, Kelloff GJ, De Flora S. Modulation of apoptosis by cigarette smoke and cancer chemopreventive agents in the respiratory tract of rats. Carcinogenesis 2001; 22: 375–80.
  • 28
    D'Agostini F, Mastracci L, Izzotti A, Balansky R, Pennisi TM, Steele VE, De Flora S. Modulation by phenethyl isothiocyanate and budesonide of molecular and histopathological alterations induced by environmental cigarette smoke in mice. Cancer Prev Res 2009; 2: 546–56.
  • 29
    Hecht SS. Chemoprevention of cancer by isothiocyanates, modifiers of carcinogen metabolism. J Nutr 1999; 129: 768S–74S.
  • 30
    Izzotti A, Bagnasco M, Cartiglia C, Longobardi M, Camoirano A, Tampa E, Lubet RA, De Flora S. Modulation of multigene expression and proteome profiles by chemopreventive agents. Mutat Res 2005; 591: 212–23.
  • 31
    D'Agostini F, Izzotti A, Balansky RM, Bennicelli C, De Flora S. Modulation of apoptosis by chemopreventive agents. Mutat Res 2005; 591: 173–86.
  • 32
    Estensen RD, Jordan MM, Wiedmann TS, Galbraith AR, Steele VE, Wattenberg LW. Effect of chemopreventive agents on separate stages of progression of benzo[alpha]pyrene induced lung tumors in A/J mice. Carcinogenesis 2004; 25: 197–201.
  • 33
    Pereira M, Tao L, Liu Y, Li L, Steele VE, Lubet RA. Modulation by budesonide of DNA methylation and mRNA expression in mouse lung tumors. Int J Cancer 2007; 120: 1150–3.
  • 34
    Balansky R, Ganchev G, Iltcheva M, Steele VE, De Flora S. Prenatal N-acetylcysteine prevents cigarette smoke-induced lung cancer in neonatal mice. Carcinogenesis 2009; 30: 1398–401.
  • 35
    Izzotti A, Balansky RM, Camoirano A, Cartiglia C, Longobardi M, Tampa E, De Flora S. Birth related genomic and transcriptional changes in mouse lung. Modulation by transplacental N–acetylcysteine. Mutat Res Rev 2003; 544: 441–9.
  • 36
    D'Agostini F, Mastracci L, Izzotti A, Balansky R, Pennisi TM, Steele VE, De Flora S. Modulation by phenethyl isothiocyanate and budesonide of molecular and histopathological alterations induced by environmental cigarette smoke in mice. Cancer Prev Res 2009; 2: 546–56.
  • 37
    Witschi H. Successful and not so successful chemoprevention of tobacco smoke-induced lung tumors. Exp Lung Res 2000; 26: 743–55.
  • 38
    Witschi H, Uyemiami D, Moran D, Espiritu I. Chemoprevention of tabacco smoke lung carcinogenesis in mice after cessation of smoke exposure. Carcinogenesis 2000; 21: 977–82.
  • 39
    Witschi H, Espiritu I. Development of tobacco smoke-induced lung tumors in mice fed Bowman-Birk protease inhibitor concentrate (BBIC). Cancer Lett 2002; 183: 141–6.
  • 40
    Witschi H, Espiritu I, Ly M, Uyeminami D, Morin D, Raabe OG. Chemoprevention of tobacco smoke-induced lung tumors by inhalation of an epigallocatechin gallate (EGCG) aerosol: a pilot study. Inhal Toxicol 2004; 16: 763–70.
  • 41
    Witschi H. Carcinogenic activity of cigarette smoke gas phase and its modulation by beta-carotene and N-acetylcysteine. Toxicol Sci 2005; 84: 81–7.
  • 42
    Kassie F, Knasmüller S. Genotoxic effects of allyl isothiocyanate and phenethyl isothiocyanate (PEITC). Chem Biol Interact 2000; 127: 163–80.
  • 43
    Ryrfeldt A, Squire RA, Ekman L. Liver tumors in male rats following treatment with glucocorticoids. Toxicol Pathol 1992; 20: 115–7.
View Full Article (HTML) Get PDF (183K)