Plasma decorin predicts the presence of esophageal squamous cell carcinoma

Authors

  • I-Chen Wu,

    1. Division of Gastroentology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Deng-Chyang Wu,

    1. Division of Gastroentology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Chun-Chi Huang,

    1. Department of Laboratory Medicine & Department of Research, Education & Training, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
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  • Hung-Shun Lin,

    1. Department of Laboratory Medicine & Department of Research, Education & Training, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
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  • Yu-Kuei Chen,

    1. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
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  • Hui-Jen Tsai,

    1. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    2. Division of Hematology/Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Chieh-Yu Lu,

    1. Division of Gastroentology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Shah-Hwa Chou,

    1. Department of Chest Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Yi-Ping Chou,

    1. Department of Chest Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan
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  • Ling-Hui Li,

    1. National Genotyping Center, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
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  • Shu-Yu Tai,

    1. Department of Family Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Ming-Tsang Wu

    Corresponding author
    1. Department of Family Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    2. Graduate Institute of Occupational Safety and Health and Research Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    3. Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
    • 100 Shih-Chuan 1st Road, Kaohsiung, Taiwan
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Abstract

Using a microarray technique, we found decorin to be underexpressed, but osteopontin (OPN) to be overexpressed, in esophageal squamous cell carcinoma (ESCC). This study aims to examine whether plasma decorin and OPN plus personal substances use (tobacco, alcohol and areca) can serve as suitable clinical markers to predict the presence of ESCC. In total, 570 archived plasma specimens (275 patients and 295 controls) were collected from 2 medical centers in Taiwan between 2000 and 2008. Decorin and OPN protein levels were measured by ELISA. Means and standard deviation of plasma decorin were 5.6 ± 3.6 ng/ml in case patients, which were significantly lower than those in controls (7.8 ± 3.1, p < 0.0001). Plasma OPN levels in case patients were not significantly different from controls (p = 0.33). When compared to subjects with the lowest quartile of plasma decorin, those with the highest quartile one had a significantly lower risk to have ESCC (Adjusted OR = 0.03, p < 0.001). Receiver operator characteristic (ROC) analysis was performed for the combination of plasma decorin and 3 substances use (smoke, alcohol and areca) for the patients compared with the controls. The area under the curve was 88.6% and the optimal cut-point of ROC curve (any 3 factors) had 73.5% sensitivity and 90.2% specificity with ∼82% of corrected classification. Plasma decorin, but not OPN, is a potential clinical marker for the detection of ESCC. When plasma decorin plus the use of the 3 substances are combined, this factor cluster could be used to detect the presence of ESCC.

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