Additional Supporting Information may be found in the online version of this article.

IJC_25571_sm_suppinfofig1.pdf35KSupplementary Fig. S1. CT16 antibody characterization. A and B, protein G-purified antibody (black squares and red circles) or antibody purified with immobilized CT16 (green and blue triangles) was diluted and exposed to a microplate coated with 0.1 μg/ml of CT16. C and D, specifity test of the antiserum. PurR, unrelated antibody to a bacterial repressor protein; Pre, preimmune serum; a2-gst, GST fusion of integrin α2 I-domain.
IJC_25571_sm_suppinfofig2.pdf79KSupplementary Fig. S2. Western analysis of cell lysates. A, CT16 detected with Protein G affinity-purified CT16 antibody. B,Western blot on the same cell lysates as in A. The membrane was washed in TBS supplemented with 0.05% Tween-20 for two days before detection with Protein G affinity-purified CT16 antibody. C, CT16 detection with CT16 antibody purified with immobilized CT16. D, determination of CT16 detection limit inWestern. Indicated amounts of recombinant CT16 was loaded on a polyacrylamide gel, and CT16 was detected with Protein G affinity-purified CT16 antibody.
IJC_25571_sm_suppinfofig3.pdf21KSupplementary Fig. S3. Kinetics of CT16 serum assay. Incubation time with the recombinant CT16-containing sample was varied while other variables were kept constant. Recombinant CT16 at concentrations of 2 ng/ml (black squares) and 200 ng/ml (red squares) was used.

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