Cancer Cell Biology

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Forced expression of Sox21 inhibits Sox2 and induces apoptosis in human glioma cells

  1. Maria Ferletta1,†,*,
  2. Demet Caglayan1,‡,
  3. Liza Mokvist1,2,‡,
  4. Yiwen Jiang1,
  5. Marianne Kastemar1,
  6. Lene Uhrbom1,
  7. Bengt Westermark1

Article first published online: 9 NOV 2010

DOI: 10.1002/ijc.25647

Issue

International Journal of Cancer

International Journal of Cancer

Volume 129, Issue 1, pages 45–60, 1 July 2011

Additional Information

How to Cite

Ferletta, M., Caglayan, D., Mokvist, L., Jiang, Y., Kastemar, M., Uhrbom, L. and Westermark, B. (2011), Forced expression of Sox21 inhibits Sox2 and induces apoptosis in human glioma cells. Int. J. Cancer, 129: 45–60. doi: 10.1002/ijc.25647

Author Information

  1. 1

    Department of Genetics and Pathology, Rudbeck laboratory, Uppsala University, SE-751 85 Uppsala, Sweden

  2. 2

    Cederroth AB, Box 715, Kanalvägen 10A, SE-194 27 Upplands Väsby, Sweden

  1. †

    Tel.: +46-18-611-11-74, Fax: +46-18-55-89-31

  2. ‡

    D.C. and L.M. contributed equally to this work.

*Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Dag Hammarskjöldsv. 20, SE-751 85 Uppsala, Sweden

  1. †

    Tel.: +46-18-611-11-74, Fax: +46-18-55-89-31

  2. ‡

    D.C. and L.M. contributed equally to this work.

Publication History

  1. Issue published online: 26 APR 2011
  2. Article first published online: 9 NOV 2010
  3. Accepted manuscript online: 7 SEP 2010 10:20AM EST
  4. Manuscript Accepted: 10 AUG 2010
  5. Manuscript Received: 7 JAN 2010

Funded by

  • Swedish Cancer Foundation
  • The Children's Cancer Foundation
  • Åke Wibergs Foundation
  • Magnus Bergvalls Foundation

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FilenameFormatSizeDescription
IJC_25647_sm_suppfig-S1.tif30368KSupplementary figure 1. Detailed human expression profiles of Sox2 and Sox21. The insilico transcriptomics (IST) database have been used to compare the mRNA expression profiles of A, Sox2, B, Sox21 20 (www.genesapiens.com). The expression profiles are divided into healthy human tissues, cancers and other diseases as indicated. Specific expression is highlighted in tissue-specific colors as indicated.
IJC_25647_sm_suppfig-S2.tif30051KSupplementary figure 2. Detailed human expression profiles of GFAP and fibronectin. The insilico transcriptomics (IST) database have been used to compare the mRNA expression profiles of A, GFAP and B, fibronectin 20 (www.genesapiens.com). The expression profiles are divided into healthy human tissues, cancers and other diseases as indicated. Specific expression is highlighted in tissue-specific colors as indicated.
IJC_25647_sm_suppfig-S3.tif27844KSupplementary figure 3. Induced Sox21 expression did not downregulate Sox2 on the mRNA level but the GFAP mRNA was reduced. The four U-343 MG-a Cl2.6 clones were treated with tetracycline (Tet) or ethanol (EtOH) up until 12 days. RNA was prepared and qRT-PCR was performed. A, Show Cl2.6 clone A1-Sox21, Cl2.6 clone A2-Sox21 and Cl2.6 clone A3-Sox21 respectively and the effect of induced Sox21 on Sox2 mRNA level. Cl2.6 clone B1-empty is the negative control clone with no induction of Sox21. B, Cl2.6 clone A2-Sox21 show the effect of induced Sox21 on GFAP mRNA level, Cl2.6 clone B1-empty is used as a negative control clone without Sox21 cDNA. Shown is one representative experiment out of 2. The values are represented as mean +/−s.d. (n=3 for each experiment).

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